摘要
目的:采用表面增强激光解析/电离飞行时间质谱技术(surface enhanced laser desorption inhibition time of flight ionization mass spectrometry,SELDI-TOF-MS)检测肝癌易发家系患者和无癌家系患者血清蛋白生物标志物,比较两者间的蛋白差异,建立筛选血清蛋白质图谱模型并初步验证.方法:选择30例肝癌易发家系患者,30例无癌家系患者,应用SELDI-TOF-MS技术及相应的计算机软件进行比较分析,检测肝癌易发家系患者血清蛋白质的特异性生物标志,建立肝癌易发家系患者的筛选模型,并对其进行了双盲法验证.结果:肝癌易发家系组与对照组共有20个蛋白质差异有显著性,以其中4个蛋白标志物(9645.48、3960.65、7895.66和5916.07 kDa),建立的筛选模型检测灵敏度为93%(28/30),特异性为90%(27/30),准确率为92%(55/60).盲法验证,灵敏度为92%(23/25),特异性为90%(18/20).结论:SELDI-TOF-MS技术具有高灵敏度和特异性,在肝癌易发家系的血清蛋白特异性生物标志物的筛选等方面具有较好的应用前景.
AIM： To detect specific serum biomarkers for liver cancer using surface enhanced laser de- sorption inhibition time-of-flight ionization mass spectrometry （SELDI-TOF-MS）, and establish a serum protein pattern for screening liver cancer prone families. METHODS： Serum patients from liver samples collected from 30 cancer prone families and 30 patients from liver cancer-free families were detected by SELDI-TOF-MS. The data were analyzed was Biomarker Wizard and BPS software to establish a serum protein pattern for screening liver cancer prone families. The pattern was evaluated by a blind test. RESULTS： A total of 20 peaks showed significant differences between the two groups, among which 4 （9645.48, 3960.65, 7895.66, 5916.07 kDa） were chosen to establish a serum protein pattern for screening liver cancer prone families. The accuracy, sensitivity and specificity of the pattern were 92% （55/60）, 93% （28/30） and 90% （27/30）, respectively. The blind test showed that the sensitivity and specificity were 92% （23/25） and 90% （18/20）, respectively. CONCLUSION： SELDI-TOF-MS has a high sensitivity and specificity in screening specific hereditary serum biomarkers for liver cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第5期690-694,共5页
World Chinese Journal of Digestology
基金
广西自然科学基金资助项目
No.2011GXNSFA018271~~
关键词
肝癌
家系
表面加强激光解吸电离-飞行
时间质谱
血清蛋白标志物
Liver cancer
Familial pedigree
Surfaceenhanced laser desorption ionization time of fightmass spectrometry
Serum biomarker
