摘要
目的探讨流式细胞术(FCM)检测CD4+T淋巴细胞内p24抗原对人免疫缺陷病毒1(HIV-1)感染的辅助诊断价值。方法通过FCM检测HIV-1感染者和正常人(阴性对照)CD4+T淋巴细胞内HIV-1 p24抗原,建立方法。收集HIV-1早期感染者样本,用FCM检测CD4+T淋巴细胞内p24抗原,ELISA检测血浆p24抗原以及nest-PCR检测核酸,比较3种方法的检测结果。结果感染者p24+CD4+T淋巴细胞比例明显高于相应阴性对照(P<0.01);感染者组p24+CD4+T淋巴细胞比例95%百分位数为1.92%,确立阴阳界值为2.00%。CD4+T淋巴细胞计数≤350个/μL的感染者p24+CD4+T淋巴细胞比例明显高于相应CD4+T淋巴细胞计数>350个/μL的感染者(P<0.05)。FCM检测HIV-1早期感染者CD4+T淋巴细胞内p24抗原结果显示,该方法的检验效能优于ELISA检测血浆p24抗原,和核酸检测相当。结论 FCM检测CD4+T淋巴细胞内p24抗原能及时发现HIV-1早期感染,在HIV-1感染的辅助诊断方面有一定的应用价值。
Objective To detect the human immunodeficiency virus 1 （ HIV-1 ） p24 antigen in CD4 ＋ T cells by flow cytometry （FCM） and assess its application for auxiliary diagnosis of HIV-1 infection. Methods CD4 ＋T cells from HIV-l-infected individuals and normal controls （negative controls） were detected for intracellular p24 antigen by FCM. Samples from early- HIV-infected individuals were collected, and the intracellular p24 antigen in CD4＋T cells was detected by FCM. P24 antigen in plasma was detected by ELISA and nucleic acids were tested by nest-PCR. The results of these 3 assays were compared. Results The rates of p24＋ CD4 ＋ T cells of the infected individuals were significantly higher than those of the negative controls （ P〈0.0l ） ; the 95th percentile of p24＋ CD4＋ T cell rates of the infected individuals was 1.92% and the cutoff value was 2.00%. Infected individuals with CD4 ＋ T ≤350 cells/μL had higher p24＋ CD4 ＋ T cell rates than those with CD4 ＋ T 〉 350 cells/ pL （P 〈 0.05 ）. The detection of p24 antigen in CD4 ＋T cells of early-HIV-l-infected patients could identify HIV-1 infection timely, and the detection efficiency of FCM was better than that of the ELISA of p24 antigen in plasma and was equal to that of nucleic acid testing. Conclusion The FCM detection of p24 antigen in CD4＋ T cells could be a promising auxiliary approach for the diagnosis of early HIV-1 infection.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第4期426-429,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
广州市医药卫生科技项目(20121A011119)
