摘要
目的探讨微小RNA-93(miR-93)对胶质瘤细胞A172增殖和凋亡的影响,观察miR-93对胶质瘤生物学行为的影响。方法通过荧光实时定量PCR(qRT-PCR)检测2例正常人脑组织、10例胶质瘤样本和5种胶质瘤细胞系中miR-93的表达;利用人工合成miR-93 mimic瞬时转染脑胶质瘤A172细胞,qRT-PCR检测细胞中miR-93的表达水平;采用MTT比色法检测A172细胞增殖情况;流式细胞术检测A172细胞周期与凋亡。结果胶质瘤样本及胶质瘤细胞系中miR-93的表达量较瘤旁与正常胶质细胞系高,miR-93 mimic上调了A172细胞中miR-93的表达水平,促进了A172细胞的增殖能力。miR-93转染细胞后,进入S期细胞显著增加,而G1期细胞则明显减少,同时A172细胞的凋亡数量减少。结论 miR-93在胶质瘤样本及胶质瘤细胞系中高表达,过表达miR-93有效促进了A172细胞的增殖,S期细胞增加G1期减少、细胞凋亡降低,提示miR-93可能成为胶质瘤诊断和治疗的新靶点。
Objective To investigate the impact of microRNA-93 on the biological behaviors of A172 glioma cells by observing the changes of cell proliferation, cell cycle and apoptosis. Methods Real-time quantitative PCR （qRT-PCR） was applied to detect the expression of microRNA-93 in 2 samples of human normal brain tissues, 10 samples of glioma tissues and 5 glioma cell lines. Artificially synthesized microRNA-93 mimic was transiently transfected into A172 glioma cells, and then the expression of microRNA-93 was tested by qRT-PCR. M＇I-I- assay was used to detect the cell proliferation of A172 glioma cells; apoptosis and cell cycle of A172 glioma cells were measured by flow cytometry. Results MicreRNA-93 was over-expressed in glioma tissues and glioma cell lines as compared with normal samples. The transient transfection of microRNA-93 mimic into A172 glioma cells significantly increased the expression of microRNA-93 in A172 glioma cells, promoted cell proliferation, raised the cell proportion in S phase, reduced the cell proportion in G1 phase, and inhibited cell apoptosis. Conclusion MicroRNA-93 was aberrantly over-expressed in glioma tissues and cell lines. Transient transfection of microRNA-93 mimic led to increased proliferation, Gl-to-S cell cycle progression and reduced apoptosis in A172 glioma cells, indicating that micro-RNA-93 might be a new target for the diagnosis and treatment of glioma.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第4期342-345,350,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81172289)
