摘要
目的 运用高效液相-高分辨飞行时间质谱（HPLC-TOF/MS）技术快速鉴别中药猫人参化学成分。方法 色谱分离采用SHISEIDO MG C18（3.0 mm×100 mm,3μm）色谱柱;流动相为乙腈和0.1%甲酸水溶液,梯度洗脱,0～10 min：10%～25%A,10～35 min：25%～90%A,35～40 min：90%A;柱温25℃;流速0.6 mL.min-1,柱后分流比为2：1。质谱定性采用飞行时间质谱,电喷雾离子源（ESI）,正离子模式,质量数扫描范围m/z 100～1 000。结果 一次性鉴别出猫人参中15种的化学成分,其中2种成分首次从猫人参中发现。结论 建立了一种基于HPLC-TOF/MS技术对猫人参中化学成分进行快速鉴别的方法,为中药猫人参的质量控制及体内的深入研究奠定了基础。
Objective To analyze the chemical constituents in Actinidia valvata Dunn. by high-performance liquid chromatography-time of flight mass spectrometry（HPLC-TOF/MS）. Methods The separation was performed on a SHISEIDO MG C18 reverse phase column（3.0 mm×100 mm, 3 μm）. The mobile phase consisted of water（containing 0.1% formic acid） and acetonitrile was used as gradient elution： 0- 10 min： 10%- 25%A; 10- 35 min： 25%- 90%A; 35- 40 min： 90%A. The flow rate was 0.6 mL·min- 1, the post-column split ratio was 2 ：1, and the temperature of column was 25 ℃. Time-of-flight mass spectrometer（TOF/MS） and electrospray ion source（ESI） were applied in qualitative analysis under positive ion mode, and mass scan range was m/z 100- 1 000. Results A total of 15 of the major chemical constituents in Actinidia valvata Dunn., including 2 new compounds, were identified by HPLC-TOF/MS. Conclusion A rapid and efficient HPLC-TOF/MS method to determine the chemical constituents in Actinidia valvata Dunn. has been established, which paves a way for the quality control and further studies of the herb in vivo.
出处
《中南药学》
CAS
2014年第2期165-168,共4页
Central South Pharmacy
关键词
猫人参
化学成分
鉴别
飞行时间质谱
Actinidia valvata Dunn.
chemical constituent
identification
TOF/MS
