摘要
目的建立太子参药材中氨基酸类成分的柱前衍生一高效液相色谱指纹图谱分析方法，为整体控制和评价太子参的质量提供依据。方法采用柱前衍生一高效液相色谱法分别对11批太子参进行检测，色谱柱为HypersilODS色谱柱（5μm，4．6mm×20cm）；流动相：醋酸钠溶液-乙腈-甲醇系统，梯度洗脱；体积流量为1．0ml·min^-1；检测波长为338nm；柱温：25℃；进样体积：1出；采用药典委员会指定的指纹图谱相似度评价软件（2004A版）进行相似度评价，计算相似度。结果建立了太子参的指纹图谱，共有12个共有峰，并达到较好分离。该分析方法具有很好的精密度、重现性和稳定性，均符合指纹图谱的技术要求。11批太子参的相似度为0．915—0．999。结论方法稳定可靠，重复性好，可为控制和评价太子参的质量提供依据。
Objective To establish pre - column derivatization - HPLC method for fingerprint analysis of amino acid in radix pseudostellaria, and to provide evidence for quality control and evaluation of radix pseudostellaria. Methods A pre - column derivatization - HPLC method was applied to detect 11 batches of radix pseudostellaria, with Hypersil ODS column as chromato- graphic column（5 μm, 4.6 mm x20 cm） at 25 ℃, and sodium acetate solution -acetonitrile -methanol as mobile phase for gradient elntion. The flow rate was 1.0 ml · min^- 1, the UV detection wavelength was at 338 nm, and the injection volume was 1 μ1. The fingerprints similarity was evaluated according to the fingerprint similarity evaluation software （2004 A） designated by the committee of pharmacopeia, and the similarity of 11 batches of radix pseudostellaria was calculated. Results The HPLC fingerprint of radix pseudostellaria was established, including 12 common peaks with better separation. This method showed good precision, repeatability and stability, which could meet the technical requirements of fingerprint. The similarity of 11 bat- ches of radix pseudostellaria was 0.915 - 0.999. Conclusion The method is reliable and reproducible, it is helpful for quality evaluation and control of radix pseudostellaria.
出处
《中国卫生检验杂志》
北大核心
2014年第4期508-511,共4页
Chinese Journal of Health Laboratory Technology
关键词
太子参
氨基酸
指纹图谱
Radix pseudostellariae
Amino acid
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