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创伤后应激障碍大鼠海马神经元凋亡和Akt／mTOR信号通路的改变 被引量：6

Alteration of Apoptosis and Akt/mTOR Signal Pathway in Hippocampal Neurons of Rat with Post-traumatic Stress Disorder
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摘要 目的检测创伤后应激障碍(PTSD)模型大鼠海马神经元凋亡和蛋白激酶B(Akt)/哺乳动物雷帕霉系靶蛋白(mTOR)信号通路的改变,探讨PTSD发病机制。方法将成年健康雄性SD大鼠60只分为对照组(n=10)和模型组(n=50)。采用改良的单一连续应激方法制备PTSD大鼠模型,在造模后1d、4d、7d、14d和28d处死大鼠(每个时间点处死5只),流式细胞术检测海马神经元凋亡率,Western blot方法检测海马第10号染色体缺失性磷酸酶张力蛋白同源物基因编码产物(PTEN)、磷酸化Akt(p-Akt)、磷酸化mTOR(p-mTOR)的表达水平。结果PTSD后1d、4d、7d和14d大鼠海马神经细胞凋亡率高于对照组(P<0.05);PTSD后1d大鼠海马PTEN蛋白表达高于对照组(P<0.05),4d达高峰,14d仍高于对照组(P<0.05);p-Akt蛋白表达水平在PTSD后1d即低于对照组(P<0.05),以后逐渐增高,28d仍低于对照组(P<0.05);p-mTOR蛋白在PTSD后4d低于对照组(P<0.05),以后逐渐增高,但28d仍低于对照组(P<0.05)。结论 PTSD模型大鼠海马神经元Akt/mTOR信号通路激活,参与海马神经元凋亡调控。 Objective To observe the changes of apoptosis and protein kinase B/the mammalian target of Rapamycin （Akt/mTOR） signal pathway in hippoeampal neurons of rat with post-straumatic stress disorder （PTSD）, and to investigate the mechanism of PTSD. Methods Sixty male adult SD rats were divided into control group （n= 10） and PTSD （n：50） model group. The PTSD animal model was established by giving the rats singleprolonged stress followed a single inescapable electric foot shock （SPS ~ S）. The neuronal apoptosis of hiappocampus of PTSD rats at 1 d, 4 d, 7 d, 14 d and 28 d after model established was detected by flow eytometry （FCM）. The expressions of phosphatase and tensin homology deleted on chromosome Ten （PTEN）, phosphorylation of ARt and roTOR （p-Akt and p-roTOR） protein were detected by Western blotting. Results The apoptotic cell rate in PTSD 1 d, 4 d, 7 d and 14 d rats were higher than that in control rats （P〈0.05）. The PTEN expression level was higher since PTSD 1 d than that in control group, and peaked in PTSD 4 d （P〈0.05）. The p- Akt expression level was lower in PTSD 1 d than that in control group, and then increased in various time points after PTSD, but it was still lower in PTSD 28 d （P〈0.05）. The p-roTOR expression level was lower than that in control group since PTSD 4 d, and then increased in various time points after PTSD 4 d, but it was still lower in PTSD 28 d （P〈0.05）. Conclusion The Akt/mTOR signal pathway was aetived in hippoeampal neurons of PTSD rats, and which was involved in neuronal apoptosis regulation.
作者 隋竹欣 刘昊 王海涛 杨占岭 刘继刚 徐爱军 卢鹤翔
机构地区 河北联合大学基础医学院组织胚胎学系 河北联合大学附属医院神经内科 遵化市人民医院口腔科遵化
出处 《四川大学学报（医学版）》 CAS CSCD 北大核心 2014年第2期221-224,共4页 Journal of Sichuan University(Medical Sciences)
基金 国家自然科学基金(No.81201048) 河北省自然科学基金(No.H2012401009)资助
关键词 创伤后应激障碍 海马 凋亡 信号通路 大鼠 Post-traumatic stress disorder Hippocampus Apoptosis Signal pathway Rats
分类号 R641 [医药卫生—外科学]
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参考文献15

  • 1North CS. Pfefferbaum B. Kawasaki A. et al . Psychosocial adjustment of directly exposed survivors 7 years after the Oklahoma City bombing. Compr Psychiatry. 2011 ; 52(1): 1-8.
  • 2Petersen T. Elklit A. Olesen JG. Victimization and PTSD in a Faroese youth total-population sample. Scand J Psychol.2010; 51( 1): 56-62.
  • 3Golub Y. Kaltwasser SF. Mauch CP. et al. Reduced hippocampus volume in the mouse model of Posttraumatic Stress Disorder. J Psycbiatr Res.2011 ;45(5) :650-659.
  • 4Schmahl C. Berne K. Krause A. et al , Hippocampus and amygdala volumes in patients with borderline personality disorder with or without posttraumatic stress disorder. J Psychiatry Neurosci . 2009; 34( 4) : 289-295.
  • 5Ding J L. Han F. Shi YX. Single-prolonged stress induces apoptosis in the amygdale in a rat model of posttraumatic stress disorder. J Psychiatr Res.2010,44(1) :48-55.
  • 6Li XM. Han F, Liu DJ. et al. Single-prolonged stress induced mitochondrial-dependent apoptosis in hippocampus in the rat model of post-traumatic stress disorder. J Chern Neuroanat , 2010 ,40( 3): 248-255.
  • 7Li X. Han F, Liu D, et al , Changes of Bax , Bcl-2 and apoptosis in hippocampus in the rat model of post-traumatic stress disorder. Neurol Res.2010,3Z(6) :579-586.
  • 8Kang S. Dong SM. Kim BR. et al . Thioridazine induces apoptosis by targeting the PI3K/ Akt/mTOR pathway in cervical and endometrial cancer cells. Apoptosis , 2012, 17 ( 9) : 989-997.
  • 9Saiki S. Sasazawa Y. Imamichi Y. et al , Caffeine induces apoptosis by enhancement of autophagy via PI3K/ Akt/mTOR/ p70S6K inhibition. Autophagy. 2011 ,7 (2) : 176-187.
  • 10Wang W. Liu Y. Zheng H. et al . A modified single-prolonged stress model for post-traumatic stress disorder. Neurosci Lett, 2008,441(2) :237-241.

二级参考文献23

  • 1Raichle ME, MacI.eod AM, Snyder AZ, et al. A default mode of brain function. Proc Natl Acad Sci USA, 2001 ,98(2): 676- 682.
  • 2Greicius MD, Flores BH, Menon V, et al. Resting-state functional connectivity in major depression: abnormally increased contributions from subgenual cingulate cortex and thalamus. Biol Psychiat,2007,62(5) :429-437.
  • 3Bluhm RL, Miller J, Lanius RA, et al. Spontaneous low- frequency fluctuations in the BOI.D signal in schizophrenic patients: anomalies in the default network. Schizophr Bull, 2007,33(4) : 1004-1012.
  • 4Bluhm RL, Williamson PC, Osuch EA, et al. Alterations in default network connectivity in posttraumatic stress disorder related to early-life trauma. J Psychiatr Neurosci,2009;34(3) : 187-194.
  • 5Buckner RL, Andrews-Hanna JR, Schacter DL. The brain's default network: anatomy, function, and relevance to disease. Ann NY Acad Sci, 2008 ,1124 (1) : 1-38.
  • 6Lanius RA, Bluhm RL, Lanius U, et al. A review of neuroimaging studies in PTSD: heterogeneity of response to symptom provocation. J Psychiatr Res,2006,40(8):709-729.
  • 7Bremner JD. Functional neuroimaging in post traumatic stress disorder. Expert Rev Neurother, 2007 ; 7 (4) : 393-405.
  • 8Moores KA. Clark CR, McFarlane AC, et al. Abnormal recruitment of working memory updating networks during maintenance of trauma neutral information in post-traumatic stress disorder. Psychiat Res, 2008 ; 163(2) : 156-170.
  • 9Broyd SJ, Demanuele C, Debener S, et al. Default-mode brain dysfunction in mental disorders: a systematic review. Neurosci Biobehav Rev, 2009 ,33(3) : 279-296.
  • 10Cavanna AE, Trimble MR. The precuneus:a review of its functional anatomy and behavioural correlates. Brain, 2006,129 (Pt3) :564-583.

共引文献11

同被引文献107

引证文献6

二级引证文献43

四川大学学报（医学版）
2014年 第2期

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