摘要
目的 通过检测终末期肝病（ESLD）患者在肝移植术前后血巨噬细胞清道夫受体（SR）-B Ⅰ、CD36的表达,探讨ESLD行肝移植术后发生动脉粥样硬化的可能机制.方法 20例行肝移植术的患者,分别于术前及术后1年记录患者血压、血脂、血糖等临床资料,观察术后1年患者发生冠心病等临床事件的情况,用逆转录-聚合酶链式反应及Western blot测定肝移植术前及术后1年血浆SR-B Ⅰ及CD36表达水平变化.结果 与术前比较,肝移植患者术后1年的血总胆固醇[（5.34±0.87） mmol/L比（4.27±0.91） mmol/L]、甘油三酯[（2.47 ±0.81）mmol/L比（1.02±0.49） mmol/L]和低密度脂蛋白胆固醇水平[（3.36±0.67） mmol/L比（2.14±0.74） mmol/L]增高（P均＜0.05）,HDL-C水平降低[（0.98 ±0.84） mmol/L比（1.58±0.34） mmol/L,P＜0.05].随访1年内,1例诊断为非ST段抬高型心肌梗死,行经皮冠状动脉介入治疗,1例诊断为心房颤动.术后1年,SR-BⅠmRNA（20.44±0.60比23.12 ±0.69,P＜0.05）和蛋白（0.21 ±0.13比0.64±0.28,P＜0.05）表达水平降低,CD36 mRNA（20.91±0.35比18.55 ±0.62,P＜0.05）和蛋白（0.94 ±0.13比0.42 ±0.19,P＜0.05）表达水平增高.结论 肝移植患者术后血SR-B Ⅰ表达水平较术前降低,CD36表达水平较术前增高,影响了肝移植术后脂类代谢,可能参与了动脉粥样硬化的发生和发展.
Objective To explore the association between expression changes of plasma maerophages scavenger receptor （SR）-B I and CD36 and risk of arteriosclerosis in end-stage liver disease （ESLD） patients post liver transplantation. Methods A total of 20 liver transplantation patients were included. Clinical data including blood pressure, blood lipid, blood glucose, incidence of new-onset cardiovascular events were obtained. Plasma macrophages scavenger receptor SR-B I and CD36 expressions were detected by polymerase chain reaction （ RT-PCR ） and Western-blot before and at 1 year after liver transplantation. Results The serum levels of TC[ （5.34 ± 0. 87 ） mmol/L vs. （4.27 ± 0. 91） mmol/Ll , TG [ （2.47 ± 0.81 ） mmol/L vs. （ 1.02 ± 0. 49 ） mmol/L 1 and LDL-C [ （ 3.36 ± 0.67 ） mmol/L vs. （ 2.14 ± 0. 74）mmol/L ]were significantly increased （P 〈0. 05） while the serum level of HDL-C [ （0.98 ±0.84） mmol/L vs. （ 1.58 ± 0.34 ） mmol/L ] was significantly reduced （ P 〈 0. 05 ） at 1 year post transplantation compared to before-transplantation levels. One patient developed non-ST segment elevation myocardial infarction and treated with percutaneous coronary intervention, another patient developed atrial fibrillation at one year after transplantation. The plasma mRNA expression of SR-B I was reduced （ 20. 44± 0. 60 vs. 23. 12 ±0. 69, P 〈0. 05） while the expression of CD36 mRNA was upregulated（20. 91 ±0. 35 vs. 18.55 ± 0. 62, P 〈 0. 05 ） at 1 year after liver transplantation compare with that of before the transplantation. Similarly, the plasma protein expression of SR-B I was reduced（0. 21 ± 0. 13 vs. 0. 64 ± 0. 28 ,P 〈 0. 05 ） while the protein expression of CD36 was upregulated （0. 94 ± 0. 13 vs. 0. 42 ± 0. 19, P 〈 0. 05 ） at 1 year after liver transplantation compare with that of before the transplantation. Conclusion Plasma expression changes of SR-B I and CD36 might contribute to the dyslipidemia and contribute to the atherosclerosis susceptibility after liver transplantation.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2014年第2期136-139,共4页
Chinese Journal of Cardiology
关键词
动脉粥样硬化
肝移植
受体
清道夫
Liver transplantation
Atherosclerosis
Scavenger receptor
