摘要
目的 探讨微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量 ,为进一步研究微波的眼底损伤机制及其防护提供一定的实验依据 .方法 体外培养猪视网膜神经节细胞 ,按微波辐照强度分为对照组 ,30 m W·cm- 2组 ,6 0 m W· cm- 2组及各辐照剂量加锌组 ,微波理疗机于微波屏蔽室内辐照 1h,辐照后立即于光镜及电镜观察细胞形态变化 ,测定超氧化物歧化酶 (SOD)和丙二醛 (MDA)活性 .结果 微波辐照后 ,细胞有聚集现象 ,部分细胞轴突消失 ,电镜可见线粒体及内质网肿胀 ,细胞 MDA活性明显增高 ,SOD降低 ,加锌各组光镜下细胞形态变化不明显 ,电镜显示线粒体轻度肿胀 ,嵴完整 ,MDA活性有所恢复 ,SOD活性增高 .结论 微波可引起视网膜神经节细胞脂质过氧化损伤 ,锌可提高细胞的抗氧化能力 。
AIM To determine the lipid peroxidation damage in the primary cultured pig retinal ganglion cells induced by microwave and cells' tolerance to microwave in order to provide some experiment foundation for study of mechanism of microwave damage and its protection. METHODS Pig retinal ganglion cells were cultured in vitro and divided into 3 groups of different radiation intensities (control group, 30 mW·cm -2 group, 60 mW·cm -2 group) and 2 more radiated groups with zinc added. The activity of intracellular superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were assayed after they had been radiated for 1h in a shielded room by 2450 MHz continuous wave physiotherapy machine. The morphological variation of cells was observed immediately after radiation by optics microscope and transmission electronic microscope. RESULTS After radiation, some cells congregated and their axon disappeared. Mitochondria and endoplasmic reticulum were detected swelling by transmission electronic microscope. The content of MDA was increased and SOD decreased clearly compared with control group. Cells had no obvious changes observed under optical microscope, but mitochondria swelling lightly and integrate mitochondria cristae by transmission could be found under electronic microscope while in zinc added groups, content of MDA recovered, and SOD increased. CONCLUSION The microwave can induce lipid peroxide damage to in vitro cultured retinal ganglion cells and zinc can improve antioxidative capacity of cells, and to some extent remedy microwave induced damage of lipid peroxidation to retina ganglion cells.
出处
《第四军医大学学报》
北大核心
2001年第1期54-56,共3页
Journal of the Fourth Military Medical University
