摘要
目的：探讨小鼠注射环磷酰胺CTX形成暂时性免疫缺陷后，在其肾筋膜接种Hela细胞，建立免疫抑制小鼠肿瘤细胞模型的可行性。方法：复苏培养Hela细胞，做软琼脂克隆形成实验，挑取生长状态良好的克隆团，分离具有较强增殖活力的细胞，取对数生长期细胞，接种（1—3）×10^7／ml的单细胞悬液0．1ml于CTX免疫抑制小鼠左侧肾上腺区肾筋膜内，接种BMEMs细胞作为阴性对照。经核磁共振成像，组织病理HE染色和免疫组化检测确认癌细胞在肾筋膜内成瘤情况。结果：39只CTX免疫抑制小鼠，荷瘤成功39只，总荷瘤率100％。结论：成功建立了CTX免疫抑制小鼠肾筋膜接种Hela细胞模型，为研究肿瘤细胞悬液在非免疫缺陷的免疫抑制机体内生长的生物学特性打下良好的基础。
Objective：To explore its feasibility that Hela cells vaccinate in the renal fascia of CTX - immunosuppressed - mice, Method： Through the soft agar clone picking Hela cells owing the strong proliferation. After proliferation, 0. 1 ml Hela cells suspension （ 1 - 3 ） ×10^7/ml was injected in CTX - immunosuppressed - mice of renal fascia in the left adrenal gland. By nuclear magnetic resonance imaging, tissue pathology HE staining and immunohistochemical detection confirm survival in the cancer cells in the renal fascia and form tumors. Result：The tumor - burdened rate was 100% which the 39 CTX immunosuppressive mice all grow tumor - burdened successed. Conclusion;Hela ceils were isolated from the soft AGAR cloning successful, and established the CTX immunosuppressive mice＇ s renal fascia vaccinated Hela cells model. It is a good foundation for the biological characteristics, for studying tumor cell suspension in the immunodeficiency immune body.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第1期75-80,共6页
Biotechnology
