摘要
紫杉醇(taxol)是主要来源于红豆杉属植物的一种天然抗肿瘤药物,紫杉烷(taxane)是紫杉醇的代谢前体或支路代谢产物,同样具有开发成为抗肿瘤新药的潜质。本文采用高效液相色谱法研究了紫杉醇(Taxol)、10-去乙酰巴卡亭Ⅲ(10-DAB)、7-木-10-去乙酰紫杉醇(7-xyl-10-DAT)、10-去乙酰紫杉醇(10-DAT)、三尖杉宁碱(CE)和7-表-10-去乙酰紫杉醇(7-epi-10-DAT)6种紫杉烷类化合物在南方红豆杉枝叶中含量的季节变化,结果显示Taxol和10-DAT在8、9月份含量最低,10-DAB和7-xyl-10-DAT在8、9月份含量相对最高,Taxol含量季节变化和10-DAB呈负相关,与CE呈显著正相关。7-xyl-10-DAT含量季节变化和10-DAB、10-DAT分别呈正相关。本文为研究南方红豆杉中紫杉醇及相关紫杉烷的代谢、积累规律提供了依据,不但有助于阐明紫杉醇的生物合成的关键步骤及调控的生理机制,而且对红豆杉资源的深度开发具有指导意义。
Taxol is a natural anticancer agent mainly extracted from Taxus species. Taxanes are the precursors or branches of taxol metabolites, also have the potential for developing as anti-tumor drugs. The seasonal change of contents of six taxanes i.e. taxol, 10-deacetylbaccatin Ⅲ（10-DAB）, 7-xylosyl-10-deacetyl-taxol （7-xyl-10-DAT）, 10-deacetyltaxol （10-DAT）, cephalomannine （CE） and 7-epi-10-deacetyl taxol （7-epi-10-DAT） in the branches and leaves of Taxus chinensis var. mairei was studied by high Performance liquid chromatography （HPLC） method. The results showed that the contents of Taxol and 10-DAT were the lowest in August and September, at the same time the contents of 10-DAB and 7-xyl-10-DAT were relatively higher. The seasonal content change of taxol was negatively correlated with 10-DAB, and positively correlated with CE. The seasonal content change of 7-xyl-10-DAT was positively correlated with 10-DAB and 10-DAT, respectively. This paper provided the theory bases for the metabolism and accumulation rules of taxol and related taxanes, it is not only helpful to clarify the biosynthesis key steps and physiological regulation mechanisms of taxol, but also has the guidance significance for Taxus resources development.
出处
《植物研究》
CAS
CSCD
北大核心
2014年第2期266-270,共5页
Bulletin of Botanical Research
基金
"十二五"国家科技支撑计划项目(2012BAD21B05)
国家自然科学基金项目(31200478
31370630)
