摘要
目的:检测梅毒血清固定患者外周血调节性T细胞(regulatory T cells,Treg)与Th17细胞的特异性转录因子及相关细胞因子mRNA水平的变化,初步探讨其在梅毒血清固定现象中的作用。方法:梅毒血清固定患者28例,同时以26例健康体检者作为对照组。采用实时定量方法检测外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中Treg和Th17细胞特异性转录因子及相关细胞因子叉状头转录因子(FOX)P3、细胞毒性T淋巴细胞抗原(CTLA)-4、白介素(IL)-10、转化生长因子(TGF)-β和孤独核受体(ROR)γt、IL-17 mRNA的表达。结果:梅毒血清固定患者外周血FOXP3、CTLA-4、IL-10、TGF-βmRNA水平的表达量明显高于正常对照组(P<0.05),RORγt和IL-17的mRNA水平表达量明显低于正常对照组(P<0.05),FOXP3/RORγt mRNA水平比值明显低于对照组(P<0.05)。结论:Treg与Th17细胞特异性转录因子及相关细胞因子mRNA水平的表达异常可能在梅毒血清固定现象形成中发挥重要作用。
Objective： To detect the changes in expression levels of transcription factors and cytokines associated with regu- latory T cells（Treg） and Th17 cells in peripheral blood mononuclear cells（PBMC） of the patients with serofast syphilis, arid to explore the possible roles of these cells in the development of serofast syphilis. Methods： Quantitative real-time PCR was used to determine the mRNA levels of FOXP3, CTLA-4, IL-IO, TGF-, RORγt and IL-17 in PBMC from 28 patients with serofast syphilis and 26 normal subjects. Results： In comparison with normal controls, patients with serofast syphilis showed higher levels of FOXP3, CTLA-4,IL-IO and TGF-β, and lower levels of RORγt and IL-17 in PBMC （P〈0.05）. The ratio of FOXP3/RORγt was higher in the patients with serofast syphilis than that in the normal controls （P〈0.05）. Conclusions： The changes in the expression levels of transcription factors and cytokines associated with Treg and Thl7 cells may take part in the pathogenesis of serofast syphilis.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2014年第3期140-142,共3页
Journal of Clinical Dermatology
基金
国家自然科学基金(30700718)
徐州市社会发展科技计划项目(XF10C057)资助项目
