摘要
目的分析乳腺癌原发灶与复发转移灶间雌激素受体（ER）表达及ER基因甲基化改变的临床意义。方法采用免疫组化法检测70例乳腺癌原发灶和复发转移灶中ER表达情况，采用甲基化特异性聚合酶链反应（MSP）检测ER基因甲基化表达的变化。结果70例乳腺癌原发灶和复发转移灶中，ER阳性率分别为64．3％和41．4％，差异有统计学意义（P〈0．05）。ER从原发灶到复发转移灶阳性转阴性患者6例（8．6％），其中4例ER基因从非甲基化状态转为高甲基化；ER从原发灶到复发转移灶阴性转阳性患者4例（5．7％），其ER基因均表现为高甲基化状态转为非甲基化状态。结论乳腺癌原发灶与复发转移灶中ER表达不一致，引起ER表达变化的原因可能与ER基因的甲基化状态有关。
Objective To investigate the different expressions of ER and ER gene status between primary and relapsed/metastatic lesions and their clinical significance. Methods ER and ER gene status of primary and relapse/metastatic breast cancer masked in 70 metastatic breast cancer patients were assessed by determination of methylation status by immunohistochemistry （IHC） and methylation specific polymerase chain reaction （MSP）, respectively. Results Positive rate of ER in the primary breast cancers was 64.3% , and in the relapse/metastatic lesions was 41.4% （ P 〈 0.05 ）. There were six patients whose positive ER status was changed to negative, among them the ER gene status was changed from demethylation to hypermethylation in four cases. Another four patients with negative ER status changed to positive, and their ER gene hypermethylation changed to ER demethylation status. Conclusions The discordance of ER expression status in primary and relapse/metastatic lesions of breast cancer might be related to DNA methylation status.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2014年第2期128-131,共4页
Chinese Journal of Oncology
基金
徐州市科技局基金项目（XM07C043）
