摘要
目的 探讨Ku86蛋白在肝细胞癌（HCC）组织中的表达及血清抗Ku86抗体在HCC早期诊断中的临床价值.方法 采用Western blot法检测HCC和癌旁正常组织中Ku86蛋白的表达,采用间接酶联免疫吸附试验（ELISA）方法检测HCC、肝硬化患者和正常对照者血清中抗Ku86抗体的水平,采用电化学发光法测定甲胎蛋白（AFP）水平.结果 HCC和癌旁正常组织中Ku86蛋白相对表达最分别为0.21 ±0.05和0.08 ±0.02,差异有统计学意义（P＜0.01）.HCC患者、肝硬化患者和正常对照者血清中抗Ku86抗体水平分别为0.47 ±0.22、0.22 ±0.06和0.11 ±0.03,与肝硬化患者比较,HCC患者血清抗Ku86抗体水平显著增高（P＜0.01）.HCC组患者中,HBV感染和HCV感染患者血清中抗Ku86抗体水平分别为0.51±0.19和0.47 ±0.24,差异无统计学意义（P =0.267）.HCC患者手术前血清抗Ku86抗体水平为0.46 ±0.16,手术后1个月和3个月的血清抗Ku86抗体水平分别为0.35 ±0.13和0.23 ±0.08,手术后血清抗Ku86抗体显著降低（P＜0.01）.抗Ku86抗体和AFP鉴别诊断HCC的受试者工作特征曲线下面积分别为0.857和0.739.HCC患者中血清抗Ku86抗体的阳性率为61.4％ （51/83）,高于AFP的阳性率（27.7％,23/83）.AFP联合抗Ku86抗体检测可将HCC检出率提高至72.3％ （60/83）.HCC患者血清中抗Ku86抗体水平与AFP水平无相关性（r=0.156,P =0.161）.结论 HCC患者血清抗Ku86抗体水平显著增高,但与HBV、HCV感染无关.抗Ku86抗体可能是早期肝癌的诊断标志物,可与 AFP互补应用于临床.
Objective To investigate the clinical value of serum anti-Ku86 in early detection of hepatocellular carcinoma （HCC）.Methods Expression levels of Ku86 protein in HCC and adjacent normal liver tissues were detected by Western blotting.Serum anti-Ku86 level in 83 patients with early HCC and 124 patients with liver cirrhosis were detected by enzyme-linked immunosorbent assay （ELISA）.Chemiluminescence was used to measure the serum level of α-fetoprotein （AFP）.Results Expression of Ku86 protein in HCC was increased when compared with the adjacent normal liver tissues （0.21 ±0.05 vs.0.08 ±0.02,P 〈0.01）.Serum anti-Ku86 level was significantly elevated in HCC patients compared with that in liver cirrhosis patients （0.47 ±0.22 vs.0.22 ±0.06 Abs at 450 nm,P 〈0.01）,but there was no significant difference between HBV infection and HCV infection in HCC patients （0.51 ±0.19 vs.0.47 ±0.24,P =0.267）.Of note,serum anti-Ku86 level was significantly decreased after surgical resection of the tumors in the 30 HCC cases tested （P 〈 0.01）.The results of ROC analysis indicated a better performance of anti-Ku86 （0.857） than AFP （0.739） for early detection of HCC.In 83 HCC patients,the positive rate of anti-Ku86 was 61.4% （51/83）,significantly higher than that of the AFP positive rate （27.7%,23/83）.The anti-Ku86 level was positive in 37 of 60 HCC cases with negative AFP.Combination assay of AFP and anti-Ku86 could detect 60 of 83 HCC cases （72.3%,60/83）.There was no significant correlation of antiKu86 and AFP （r =0.156,P =0.161）.Conclusions Serum anti-Ku86 level is significantly elevated and is not related to HBV and HCV infection in HCC patients.Serum anti-Ku86 antibody may be a potential biomarker for early detection of HCC,and can be used in combination with AFP in clinics.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2014年第2期123-127,共5页
Chinese Journal of Oncology
关键词
肝肿瘤
抗Ku86抗体
甲胎蛋白
诊断
Liver neoplasms
Anti-Ku86 antibody
Alpha-fetoprotein
Diagnosis
