摘要
目的研究血小板第 ４因子（ＰＦ４）对６０Ｃｏ γ射线辐射损伤小鼠骨髓的造血防护作用及其机制。方法小鼠腹腔注射ＰＦ４后间隔 ６ ｈ第 ２次注射； ２０ ｈ后，给予 ７． ５Ｇｙ的６０Ｃｏ γ射线照射 １次（全身照射），观察第８天骨髓细胞ＤＮＡ含量的变化。结果照射后８ｄ，ＰＦ４处理组小鼠的骨髓有核细胞中ＤＮＡ含量及细胞周期各时相，与照射组相比较有明显差异（Ｐ＜０．０１），而与对照组基本相同（Ｐ＞０．０５）。结论ＰＦ４能保护小鼠骨髓造血干细胞免受电离辐射引起的损伤。
Aim To investigate radioprotective effects of platelet factor 4 (PF4) on hemopoiesis of mice after γ-ray irradiation and their mechanisms. Methods The mice were injected with PF4 twice at 6h intervals; and 20 h after the second injection they were given one irradiation of 7. 5Gy 60Co γ-ray. 8d later the colony forming hot of granulocyte - macrophage (C F U - GM ), colony forming of unit spleen (FU-S), number and DNA contents in bone marrow cells(BMCs) were examined by flow cytometry. Results 8d after irradiation, a significant difference was shown between DNA contents in BMCs and cell cycles of the PF4-injected mice and those of the irradiated mice (P< 0. 01), whereas little difference was found between those of the irradiated mice and the controls (P>0. 05). Conclusion PF4 can obviously protect hemopoietic stem cells (HSCs) of mice from radiation injury and promote proliferation of HSCs after radiation.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第1期46-47,共2页
Chinese Journal of Cellular and Molecular Immunology
