摘要
目的 :通过检测衰老大鼠肝组织细胞线粒体呼吸链氧化功能 ,探讨大鼠衰老时肝组织细胞线粒体呼吸链氧化功能的变化规律及 94 1方延缓衰老的作用机制。方法 :分别从 4月龄 (青年组 )、 2 4月龄 (老龄组 )和 94 1方组大鼠的肝组织分离出线粒体 ,并用双缩脲法测定单位组织的线粒体蛋白 ,用Clark氧电极极谱法分别测定线粒体呼吸链 3个氧化酶活性 :NADH氧化酶(NADHOX)、琥珀酸氧化酶 (SUCOX)和细胞色素氧化酶 (CYTOX)。用差光谱法 (联二亚硫酸钠还原减正铁氢化钾氧化的消光值 )获得细胞色素a、b、c和c1含量 (Cyta、Cytb、Cytc和Cytc1)。结果 :与青年组大鼠比较 ,老年组大鼠的肝组织细胞线粒体含量有一定下降 ,而 3个氧化酶活性却显著增加 ,同时细胞色素含量尤其是Cytb和Cytc显著升高。 94 1方组大鼠肝组织细胞线粒体上述指标皆显著高于青年组和老年组。结论 :衰老大鼠肝组织线粒体酶活性与青年组有显著不同 ,表明衰老过程中肝细胞通过某种反馈机制使残存完好的线粒体氧化功能代偿性增强 ;94 1方使这种作用进一步增强。目的 :通过检测衰老大鼠肝组织细胞线粒体呼吸链氧化功能 ,探讨大鼠衰老时肝组织细胞线粒体呼吸链氧化功能的变化规律及 94 1方延缓衰老的作用机制。方法 :分别从 4月龄 (青年组 )、 2 4月龄 (?
Objective:To study functional changes in the activity of respiratory chain enzyme of mitochondrion isolated from the liver of aged rats and the effects of 941 Recipe(941R,a traditional medicine formula)on it.Method:The liver mitochondrion were isolated from rats aged 4 months(young group),24 months(senescent group)and 941 group respectively by differential centrifugation;the determination of mitochondria population in rat hepatic cell was obtained quantitatively from measurement of mitochondria protein by a bruit method;NADH oxides (NADHOX),succinct oxides(SUCOX),and the content of cytochrome a,b,c,and c 1(Cyt a,b,c and c 1)were evaluated by the differential spectra(dithionite-reduced minus ferricyanide-oxidized).Result:In comparison with the young group,in senescent rats there was a decline in mitochondria population;The activities of these oxidizes,however,were significantly increased,and the content of cytochrome,especially Cyt b,c 1,was markedly increased;the parameters cited above in 941R group were significantly elevated over those in young and senescent rats.Conclusion:These results suggest that the enhancement of respiratory enzymatic activities of the liver mitochondrion from aged rats appears to be some compensatory mechanism by witch increase workload of the remaining intact population of mitochondrion,and 941R more efficiently improves the compensatory mechanism.
出处
《安徽中医学院学报》
CAS
2001年第1期35-37,共3页
Journal of Anhui Traditional Chinese Medical College
