摘要
目的 应用组织工程学方法重建颞下颌关节盘软骨。 方法 分离 6只日本大耳白兔髁状突软骨细胞 ,进行细胞的微载体大规模扩增 ,将扩增后的软骨细胞接种于组织引导再生胶原膜 ,体外适当培养后植入 4只同种成年兔皮下。植入后 12周 ,对所获组织进行组织形态学观察。 结果 髁状突软骨细胞在胶原膜内生长良好 ,植入动物体内 12周后可形成乳白色类软骨样组织 ,其表面光滑 ,有弹性。甲苯胺蓝染色 ,细胞周围基质呈异染性。 结论 应用胶原膜结合软骨细胞共同培养 ,可形成软骨样组织。
Objective To reconstruct the temporomandibular joint disk cartilage with tissue engineering in rabbits. Methods The mandibular condylar cartilage (MCC) cells were harvested from 6 Japan white rabbits with enzymatic method. They were augmented extensively in a micro carrier culture system. The augmented MCC cells were inoculated into collagen films of guided tissue regeneration, and they grew in vitro for a week. After that, the cell collagen complex was transplanted subcutaneously into the backs of 4 Japan adult white rabbits. After 12 weeks of the transplantation, the rabbits were sacrificed and the newly fabricated cartilaginous tissues were extracted. The samples were assessed histomorphologically. Results The MCC cells could attach and grow well into the collagen films in vitro, and ivory cartilage like tissues with smooth surface and elasticity formed after 12 weeks of the transplantation. Stained with toluidine blue, the stroma around the cells showed metachromia. Conclusions Cartilage like tissues can be formed with the coculture of collagen films and cartilage cells, which will be an effective way for repairing cartilage defects and temporomandibular joint disk damage.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2001年第1期20-22,共3页
Chinese Journal of Trauma
基金
国家自然科学基金资助项目!(39770 80 0 )
关键词
颞下颌关节备用软骨重建
组织工程
软骨缺损
Temporomandibular joint
Mandibular condylar
Cells, cartilage
Tissue engineering
