摘要
研究对不同栽培模式(净栽和混栽)下采集的113份感染小麦条锈病的新鲜叶片,从罹病叶片中直接提取罹病组织的DNA,利用我国及云南省的5个小麦条锈菌主要流行小种(条中32,条中29,条中31,条中23和水源类型)特异的分子标记对小麦条锈菌生理小种进行分子检测,明确不同栽培模式对小麦条锈菌群体结构的影响。结果表明,混栽条件下主要小种特异标记的检出率比净栽的低,净栽田块的优势小种特异标记为条中32和条中29,出现频率分别为81.5%和78.5%,而混栽田块的优势小种特异标记为条中29和水源类型,出现频率分别为41.7%和18.8%;混栽田块中41.7%的叶片中含有2个以上小种特异标记,58.3%的叶片中含有1个小种特异标记,而净栽田块中75.0%的叶片中含有2个以上小种特异标记,25.0%的叶片中含有1个小种特异标记。表明小麦品种混栽能有效地减少单个叶片中的小种数,并显著降低小麦条锈菌优势小种的出现频率,前者可能是减少病害严重度的重要原因,而后者则可能对病害的流行产生重要影响。
In order to explicit the effects of the population struction of Puccinia striiformis f. sp. tritici under different cultivation patterns （mix-cultivation and monocuhivation） in the fields. Five race-spe- cific-markers （CY32, CY31, CY29, CY23 and Shuiyuan pathogype） were used to survey 113 dis- eased samples collected from two cultivation patterns. The results indicated that dominant race-specif-it-markers were higher frequency under mono-cuhivation than that of mix cultivation, the dominant race-specific-markers were CY32 and CY29 under mono-cuhivation, the frequency of detection were 81.5% and 78.5%, respectively. The dominant race-specific-markers were CY29 and Shuiyuan pathotype under mix cultivation, the frequency of detection are 41.7% and 18.8%, respectively. The several race-specific-markers were detected in single infected leaf and 41.7% infected single leaf de- tected with more than two race-specific-markers, 58.3% infected single leaf detected with one race- specific-markers under mix cultivation pattern, while there were 75.0% infected leaves with more than two race-specific-markers, 25.0% infected single leaf detected with one race-specific-markers under mono-cuhivation pattern. The results indicated that the mix cultivation pattern can reduce races on sin- gle leave and suppress the frequency of occurrence of dominant races of P. striiformis f. sp. tritici in the fields significantly, subsequently reduced the servility and prevalence of the disease.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第1期11-15,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
农业部小麦条锈病行业专项基金项目(200903035-7)
云南省重点基金项目(2009CC004)
关键词
小麦条锈菌
生理小种
分子检测
小种特异标记
栽培模式
Puccinia striiformis f. sp. tritici
race
molecular detection
race-specific-marker
culti-vation pattern
