摘要
以温敏雄性不育系K121S在可育温度（10℃）下和不育温度（26℃）下三核期花蕾为材料,以可育温度下的cDNA为驱动子,通过抑制性差减杂交技术构建了不育温度下抑制性差减杂交（SSH）文库,其插入片段长度分布在200～1 000 bp左右,并随机挑选97个阳性菌落测序,得到20个表达序列标签（EST）,包括与ATP合酶β亚基、Rubp羧化酶小亚基同源的EST等,为进一步获得育性转换相关基因和揭示K121S的育性转换分子机理奠定了基础.
In this study, using flower-bud at thinucleate stage from thermo-sensitive male-sterile line K121S （Brassica juncea） at fertile temperature and sterile temperature as material, SSH library was constructed with driver cDNA obtained at male-fertile temperature. Ninety-seven clones were chosen randomly in sequencing. Twenty EST were discovered analogous to genes such as ATP synthetase sub- unit beta, ribulose-1, 5-bisphosphate carboxylase small subunit. The size of the DNA inserted ranges from 200 to 1 000 bp. The library laid the foundation of obtaining of the genes for fertility conversion and clarification of molecular mechanism of K121S fertility conversion.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第1期6-10,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(31160289)
关键词
油菜
温敏雄性不育
抑制性差减杂交
文库
rapeseed
thermo-sensitive male sterility
suppression subtractive hybridization
library
