摘要
目的:评价DAPK、FHIT基因启动子区甲基化状态在大肠癌发生发展中的作用和临床意义。方法:采用甲基化特异性聚合酶链反应(methylation-specific PCR,MSP)检测23例大肠癌及相应癌旁正常组织的DAPK、FHIT基因启动子区甲基化状态。结果:大肠癌组织的DAPK、FHIT基因甲基化率分别为60.86%(14/23)、52.17%(12/23),高于癌旁正常组织的13.04%(3/23)、8.69%(2/23),组间差异均有统计学意义(P<0.05),DAPK、FHIT基因甲基化状态与分化程度、淋巴结转移有显著相关性(P<0.05),与性别、年龄、肿瘤部位、Dukes分期无相关性(P>0.05)。结论:DAPK基因和FHIT基因启动子区的高甲基化可能与大肠癌的发生、发展、预后有关。
Objective： To evaluate the role and clinical significance of the methylation of the deathassociated protein kinase （DAPK） gene and fragile histidine triad （FHIT） in colorectal cancer. Methods： Methylation-specific PCR （MSP） was used to test methylation patterns of DAPK and FHIT gene in 23 colorectal cancer tissues and corresponding adjacent normal tissues. Results： Ab- errant methylation rates of DAPK and FHIT were detected in colorectal cancers （60.86%, 52.17% ） , which were significant higher than that in adjacent normal tissues（ 13.04% , 8.69% ）. The methylation status of DAPK and FHIT gene was associated with differentiated degree and lymph node metastasis （P 〈 0.05） , but not associated with age, gender, tumor site and Dukes stage （P 〉 0.05）. Conclusion ： The aberrant promoter methylation of DAPK and FHIT may be related with occurrence, development and clinical prognosis of colorectal cancer.
出处
《河南医学研究》
CAS
2014年第1期4-7,共4页
Henan Medical Research
