摘要
以草莓新品种晶玉为试材,研究了不同植物生长调节剂浓度和配比对其叶片、叶柄再生的影响及不同暗培养时间对叶片再生的影响,并筛选出了最佳的生根培养基,建立了晶玉的高效离体再生体系。结果表明,培养基MS+2.0 mg/L TDZ+0.05 mg/L 2,4-D对晶玉叶片不定芽的分化效果最好,平均不定芽再生率可达81.45%,每叶块平均再生芽数达到4.47个;暗培养7 d有利于叶片不定芽的再生。培养基MS+3.0 mg/L TDZ+0.05 mg/L 2,4-D对晶玉叶柄不定芽再生的效果最好。最佳生根培养基为1/2 MS+0.05 mg/L NAA+0.05 mg/L IBA。
Using the strawberry variety Jingyu as material,the effects of concentration and ratio of plant growth regulators on the regeneration of leaves and petiole were studied.The effects of different dark culture time on the regeneration of leaves were studied.The best rooting medium was screened,A high efficient system of in vitro regenerating Jingyu was established.The results showed that the optimal bud differentiation medium of the leaves from Jingyu was MS＋2.0 mg/L TDZ＋0.05 mg/L 2,4-D.The average adventitious bud regeneration rate was 81.45％ and the average regeneration bud number was 4.47 per leaf discs.Dark culture treatment of seven days could increase the rate of adventitious bud regeneration.For Jingyu petioles,the optimal adventitious bud regeneration medium was MS＋3.0 mg/L TDZ＋0.05 mg/L 2,4-D.The best rooting medium of strawberry Jingyu was 1/2 MS＋0.05 mg/L NAA＋0.05 mg/L IBA.
出处
《湖北农业科学》
北大核心
2013年第23期5912-5916,共5页
Hubei Agricultural Sciences
基金
国家农业科技成果转化项目(2012GB2D100298)
湖北省农业科技创新中心资助项目(2007-620-001-03)
武汉市青年科技晨光计划项目(201150431127)
关键词
草莓
晶玉
离体再生
叶片
叶柄
strawberry
Jingyu
in vitro regeneration
leaf
petiole
