摘要
广两优476是广占63-4S和R476配组而成的两系中籼新组合,其父本R476是利用分子标记辅助选择技术育成的含有抗褐飞虱基因Bph14的恢复系。根据水稻抗褐飞虱基因Bph14的序列设计显性标记76-3用于鉴定广两优476的种子纯度。结果表明,用显性标记鉴定两系杂交稻的种子纯度,方法简单、结果可靠。
Guangliangyou 476 is a new two line mediun-indica rice hybrid of crossing Guangzhan 63-4S（as sterile line） with R476 （as restorer line）.R476 is a restorer line carrying the rice brown planthopper resistant gene Bph14 bred by molecular marker-assisted selection.A dominant marker 76-3 was developed from the sequence of Bph14 gene and employed to identify seed purity of Guangliangyou 476.The results showed that it provided a feasible and reliable method for using dominant marker to identify seed purity of two-line rice hybrid.
出处
《湖北农业科学》
北大核心
2013年第23期5884-5886,5905,共4页
Hubei Agricultural Sciences
基金
农业科技成果转化资金项目(2010GB2D100297)
关键词
显性标记
广两优476
纯度鉴定
dominant marker
Guangliangyou 476
purity identification
