摘要
目的观察电针对阿尔茨海默病(AD)大鼠细胞凋亡相关蛋白Bcl-2、Bax的影响,探讨针灸治疗AD的作用机理。方法将大鼠随机分为正常组、假手术组、模型组、电针组,双侧海马注入Aβ25-35建立AD大鼠模型,造模成功后,电针组选取双侧"肾俞"、"内关"、"大椎",接韩式疼痛治疗仪治疗,Morris水迷宫检测各组大鼠学习记忆能力的变化;免疫组化法检测大鼠海马区Bcl-2、Bax的平均光密度表达。结果通过2个疗程的治疗,电针组和模型组大鼠海马区Bcl-2的表达明显低于正常组和假手术组(P<0.05),且电针组明显高于模型组(P<0.05);电针组和模型组大鼠海马区Bax表达高于正常组和假手术组(P<0.05),且电针组明显低于模型组(P<0.05)。结论电针可以改善AD模型大鼠的学习记忆能力,其作用机制可能与调节细胞凋亡相关蛋白Bcl-2、Bax有关。
Objective To observe of electro - acupuncture on rat neuronal apoptosis related protein Bcl - 2, Bax impact of Alzheimerg disease （Alzheimer＇s disease, AD）, in order to investigate the mechanism of acupuncture in treatment of AD. Methods Wistar rats were randomly divided into four groups ： normal group, sham - operation group, model group , electro - acupuncture and moxibustion group. AD rats were fabricated by injecting AI325 - 35into the double hippocampus, the treatment group stimulate＂ DaZhui＂, ＂NeiGuan＂ ,＂ Shenshu＂ u- sing electroacupuncture,immunohistochemistry was used to determine the levels of expression of Bcl -2 ,Bax in rat hippoeampi area. Results After two courses of treatment, compared with the normal group and sham group, the expression of the Bcl - 2 increased in rat hippoeampi area on model group（ P 〈 0.05 ）, compared with the model group, the treatment group decredsed obviously （ P 〈 0.05 ） ; and compared with the normal group and sham group, the expression of the Bax decreased evidently on model group（ P 〈 O. 05 ） , compared with the model group, the treatment group incredsed evidently（P 〈 O. 05 ）. Conclusion Neuronal apoptosis protein acupuncture can improve the AD model rats, and its mechanism may be related to the regulation of neuronal apoptosis related protein Bcl - 2, Bax.
出处
《湖北中医药大学学报》
2014年第1期24-26,共3页
Journal of Hubei University of Chinese Medicine
基金
国家自然科学基金(81273864
20121607)
