摘要
目的:探讨杀伤细胞免疫球蛋白样受体(Kill cell immunoglobulin-like receptor,KIR)与人类白细胞抗原(Human leukocyte antigen,HLA)组成的受体-配体基因对与桥本甲状腺炎(Hashimoto’s thyroiditis,HT)的关联性。方法:选择散发HT患者44例和健康对照43例,提取全血基因组DNA,序列特异性引物聚合酶链反应检测4个KIR基因和8个HLA-C基因,根据受体-配体特异识别原则组成KIR2DL1+HLA-C2、KIR2DL2/2DL3+HLA-C1、KIR2DS1+HLA-C2和KIR2DS2+HLA-C1共4对KIR/HLA-C基因对,分析各基因对频率在两组人群中的差别。结果:活化性KIR2DS2+HLA-C1基因对在HT患者组与对照组频率差异有显著性(36.36%vs 16.28%,P<0.05)。其余基因对在两组间未见明显差异。结论:HT患者活化性KIR/HLA-C基因对频率升高,传递活化性信号活化NK细胞和T细胞,可能是HT发病的免疫遗传学因素之一。
Objective： More research have been carried out to demonstrate that the gene pair consisted by kill cell immunoglobulin-like receptor （KIR） and human leukocyte antigen （HLA） played a role in the pathogenesis of many immunologic diseases. The aim of this study is to investigate the relationship of certain KIR/HLA-C gene pair with Hashimoto＇ s thyroiditis （HT）. Methods： For- ty-four HT patients and forty-three randomly matched healthy controls were enrolled to detect the KIR and HLA-C genotypes. The ge- nomic DNA were extracted, followed by four selected KIR genes and eight HLA-C genes were detected, and all of the target genes were determined by a polymerase chain reaction using sequence-specific primers （PCR-SSP）. The four KIR genes were KIR2DL1, KIR2DL2/3, KIR2DS1 and KIR2DS2/3, as well as eight HLA-C consisted of HLA-Cw01-08 genes. Then the frequencies of four gene pairs, KIR2DL1 ＋ HLA-C2, KIR2DL2/3 ＋ HLA-C1, KIR2DS1 ＋ HLA-C2 and KIR2DS2 ＋ HLA-C1, were compared between HT patients and healthy control individuals. Results： The frequency of KIR2DS2/HLA-C1 gene pair was significantly higher of the patient group than that of the control group （36.36% vs 16.28% ,P 〈 0.05 ）. There were no significant differences in the frequencies of other KIR/HLA-C gene pairs. Conclusion： The increased frequencies of KIR2DS2 and HLA-C1 gene pair might confer to the pathogenesis of HT disease.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第1期110-113,共4页
Chinese Journal of Immunology
基金
国家自然科学基金项目(30901461)
