摘要
微生物群落多样性的研究方法随着现代生物学技术经历了几代发展,基于形态学、生理学、分子生物学的传统研究方法在取得大量研究成果的同时,受对环境微生物群落多样性认识的限制,亟待获得一种能更加全面、准确、快速反映环境微生物群落结构的方法。本文首先回顾了传统研究方法,重点介绍了高通量测序技术,包括研究者所关注的测序仪分布、类型、原理、优势与制约,列举了多样性研究中的分析作图软件,最后以454的焦磷酸测序为例具体阐述了该方法在分析水环境微生物群落多样性中的应用。
The traditional research methods of the diversity of microbial communities based on morphology, physiology and molecular biology had gain significant achievements, however, a comprehensive accurate and rapid method to reflect the diversity of microbial communities is needed urgently due to their limitation for knowledgment of the diversity. In this paper, the traditional research techniques and the high throughput sequencing techniques including the distribution, classification, principle, advantages and limitations were summarized. Then some analyzing and plotting software were introduced, finally the application of 454 pyrosequencing to study the diversity of water microbial communities was elaborated.
出处
《化学通报》
CAS
CSCD
北大核心
2014年第3期196-203,共8页
Chemistry
基金
上海城投原水有限公司"基于物联网的青草沙水库藻类监测预警平台研究"资助
