摘要
目的探讨人脐带间充质干细胞(UCMSCs)与骨髓间充质干细胞(BMMSCs)在体外对造血干细胞的支持作用。方法分别从人脐带和骨髓中分离、培养间充质干细胞,通过免疫细胞化学染色等方法对其进行表型鉴定;采用流式细胞仪测定脐血单个核细胞的周期分布,采用甲基纤维素法测定脐血单个核细胞混合集落形成单位(CFU-Mix),比较UCMSCs和BMMSCs对脐血单个核细胞细胞周期、CFU-Mix形成能力的影响。结果成功培养获得UCMSCs和BMMSCs,鉴定结果符合预期;与非共培养组细胞相比,UCMSCs和BMMSCs共培养均能促进脐血单个核细胞进入增殖周期,并增加其形成CFU-Mix的能力(P<0.05),但UCMSCs和BMMSCs共培养组之间比较差异无统计学意义(P>0.05)。结论成功从人脐带和骨髓组织中培养获得间充质干细胞,两种来源的间充质干细胞均能提高脐血单个核细胞的体外增殖能力及CFU-Mix形成能力,均具有造血支持作用。
Objective To explore the supportive effects of mesenchymal stem cells （MSCs）derived from hu-man umbilical cord （UCMSCs）and bone marrow （BMMSCs）on hematopoiesis in vitro.Methods MSCs were isola-ted from umbilical cord and bone marrow,and the purity of MSCs was analyzed by immunocytochemical stain.The distribution of mononuclear cell cycle was determined by flow cytometry.Half-solid methylcellulose was used to de-termine the colony forming unit-mixture （CFU-mix）of umbilical cord blood mononuclear cells.Influence of UCM-SCs and BMMSCs on cell cycle and forming ability of CFU-Mix among umbilical cord blood mononuclear cells were compared.Results UCMSCs and BMMSCs were both successfully isolated and identified.Compared with the blank control group,there were more umbilical cord blood mononuclear cells in S＋G2＋M phase and CFU-mix after inter-vening with UCMSCs and BMMSCs （P＆lt;0.05）,but the difference between UCMSCs and BMMSCs was not signifi-cant （P＆gt;0.05）.Conclusion UCMSCs and BMMSCs were successfully isolated and identified,both of which could in vitro improve the proliferation and CFU-Mix forming ability of umbilical cord blood mononuclear cells,and might be able to support hematopoiesis.
出处
《检验医学与临床》
CAS
2014年第4期433-435,共3页
Laboratory Medicine and Clinic
基金
浙江省教育厅科研项目课题(Y201226068)
关键词
脐带
骨髓
间充质干细胞
体外
脐血单个核细胞
细胞增殖
umbilical cord
bone marrow
mesenchymal stem cells
in vitro
umbilical cord blood mononuclear cells
proliferation
