摘要
目的建立高效液相色谱法同时测定菊花中绿原酸、黄芩苷和槲皮素的含量。方法采用Hypersil ODSC18柱（4．6mm×250mm，5μm），甲醇-体积分数0．4％乙酸溶液为流动相，梯度洗脱，流速1．0mL·min-1，检测波长为337nm，柱温25℃。结果绿原酸、黄芩苷和槲皮素均能达到基线分离，且有较宽的线性范围和良好的线性关系（r〉0．9995）；回收率均在99．2％-99．4％内。结论该方法简便准确，稳定好，可用于同时测定菊花中绿原酸、黄芩苷和槲皮素的含量。
Objective To establish an HPLC method for the determination of chlorogenic acid, baicalin, and quercetin in the Chrysanthemi Flos. Methods A hypersil ODS C18 column （ 4.6mm×250mm,5μm） was used with methanol-0. 4% acetic acid solution as mobile phase. Gradient elution programme was ap- plied with the flow rate of 1.0 mL. min-1. The detection wave length was set at 337 nm and the column temperature was 25 ℃. Results Chlorogenic acid, baicalin and quercetin were separated perfectly with wide linear range and good linear relationship （ r 〉 0. 999 5 ） ; average recovery were 99.2%-- 99.4%. Conclusions This method is simple and accurate, and the results are stable. It can be used to determine the contents of chlorogenic acid,baicalin and quercetin in Chrysanthemi Flos.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2014年第2期112-115,共4页
Journal of Shenyang Pharmaceutical University
基金
国家自然科学基金资助项目(21002015)
关键词
高效液相色谱法
绿原酸
黄芩苷
槲皮素
菊花
含量测定
HPLC
chlorogenic acid
baicalin
quercetin
Chrysanthemi Flos
content determination
