摘要
目的 :探讨一氧化氮 -环磷酸鸟苷 (NO- c GMP)通路在支气管哮喘发病机制中的作用。方法 :给 10名健康人、13例缓解期哮喘病人及 2 9例发作期哮喘患者诱导痰液 ,检测诱导痰液中 NO2 - /NO3- 及 c GMP的水平。并对其中 11例哮喘发作期患者应用强的松 (30 mg/d) ,治疗 1周的前后进行自身对照研究。结果 :哮喘发作期患者 NO2 - /NO3-水平显著高于健康对照者 [(4 0 6 .34± 5 12 .18) μmol/L,(71.80± 10 0 .98) μmol/L,P<0 .0 1],应用激素后患者在症状、体征好转的同时 ,NO2 - /NO3-和 c GMP的水平均有明显下降 ,稳定期哮喘患者的 NO2 - /NO3-水平同正常人相比差异无显著性。结论 :NO- c GMP通路可能在哮喘的发病中起重要作用 ,诱导痰 NO2 - /NO3- 的测定是无创伤性检测哮喘气道炎症的一项简便易行的实用方法。
Objective[WT5,5”BZ]:The study was designed to investigate the role of nitric oxide (NO) in the pathogenesis of asthma.Methods:In this study, sputum was induced with nebulized hypertonic saline in ten healthy, thirteen asymptomatic and twenty nine symptomatic asthmatic subjects. The concentrations of stable end products of NO, nitrite and nitrate (NO 2 -/NO 3 -), cGMP in induced sputum were examined. We also studied the effects of oral predisone (30 mg daily for a week) in eleven symptomatic asthmatics. Results:Because of chest tightness, all subjects, but one as the chest tightness, finished the sputum induction. The concentrations of NO 2 -/NO 3 - in induced sputum were significantly higher with symptomatic asthmatics than in normal control subjects [(406.34±512.18) μmol/L, (71.80±100 98) μmol/L, P<0.001 ]. Administration of oral predisone (30 mg) resulted in an improvement of symptom and a fall in the level of NO 2 -/NO 3 - and cGMP. Conclusions:NO cGMP may play an important role in asthma. The measurement of NO derivatives in induced sputum may be useful for assessing allergic inflammation in airway and the effect of anti inflammation therapy. [
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第1期19-20,23,共3页
Journal of Nanjing Medical University(Natural Sciences)
