摘要
目的探讨不同浓度藤黄酸（gambogicacid，GA）对结肠癌LoVo细胞株增殖、侵袭能力及基质金属蛋白酶．2（MMP．2）蛋白表达的影响。方法CCK-8法检测藤黄酸对LoVo细胞增殖的影响；Transwell小室检验LoVo细胞体外侵袭能力的改变；WestemBlot检测LoVo细胞中MMP．2蛋白的改变。结果CCK-8检测显示，GA剂量1、2、4、6、8mol／L作用于细胞24h、48h后，对结肠癌Lovo细胞增殖的抑制率分别为（0．16±0．11）％、（0．24±0．08）％、（0．58±0．01）％、（0．67±0．03）％、（0．79±0．01）％和（0．27±0．05）％、（0．69±0．09）％、（0．85±O．01）％、（0．87±0．01）％、（0．89±0．01）％，与对照组（0~tmo／L）比较差异具有统计学意义（P〈O．05）。Transwell侵袭实验显示，低剂量藤黄酸可抑制LoVo细胞侵袭，其中对照组（Ortrno／L）穿膜细胞数为（120．50±8．69）个，实验组的（1ixmol／L）穿膜细胞数为（69．83＋--4．75）个，两组比较差异有条件下意义（P〈0．05）。WesternBlot结果显示，GA剂量1、2、4p,mol／L作用LoVo细胞后MMP．2蛋白表达水平分别下降48．67％、74．72％、82．70％，与对照组（0gmo／L）比较差异有统计学意义。结论藤黄酸可抑制LoVo细胞增殖及其侵袭能力，其作用机制可能与降低LoVo细胞中MMP．2蛋白表达有关。
Objective To investigate the effects of gambogic acid on proliferation, invasion and protein expression of MMP-2 in LoVo human colon cancer cell line. Methods The CCK-8 assay was used to determine the anti-proliferation ability. Transwell chamber assay was used to study the invasion of LoVo colon cancer cells. Western Blot was applied to examine the protein expression of MMP-2. Results CCK-8 assay showed that after cells treated with 1, 2, 4 μmol/L GA for 24 h, 48 h the inhibition rates were （0.16 ± 0.11）%, （0.2±0.08）%, （0.58±0.01）%, （0.67_0.03）%, （0.79±0.01）% and （0.27±0.05）%, （0.69±0.09）%, （0.85±0.01）%, （0.87±0.01）%, （0.89±0.01）%, and there had statistically significant differences between control group （0 μmo/L） with experimental group （P〈0.05）. Gambogic acid can effectively inhibit LoVo cells invasion at a low-dose concentration, which the numbers of cells that digested the matrigel in control group （0 gmo/L） was 120.50 ± 8.69 and 69.83 ±4.75 in experimental group （ 1 pmol/L） , P〈0.05） . Western Blot revealed that Gambogic acid can down-regulate the expression of MMP-2, which the levels of down-regulating the expression of MMP-2 after treated with 1, 2, 4 umol/L GA were 48.67%, 74.72%and 82.70% （P〈0.05） Conclusion Gambogic acid can effectively inhibit the growth and invasion of the LoVo cells, which may be related with down-regulating the expression of MMP-2.
出处
《国际中医中药杂志》
2014年第3期220-222,共3页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目（项目编号：No.81272556）
广东省医学科研基金项目（项目编号：A2005543）
