摘要
目的 观察全脑缺血再灌注时脑组织抗凋亡基因 bcl－ 2蛋白表达及降钙素基因相关肽 (CGRP)对 bcl－ 2蛋白表达的影响。方法 应用颈动脉负压分流法制备大鼠全脑缺血再灌注模型，应用免疫组织化学法检测全脑缺血 30min再灌注 3h、 6h以及经颈动脉注射 (CGRP)后再灌注 3h、 6h大脑皮层和海马区 bcl－ 2蛋白的表达。结果 大鼠全脑缺血 30min再灌注 3h、 6h大脑皮质及海马 bcl－ 2蛋白表达明显增加，应用 CGRP再灌注 3h、 6h大脑皮质及海马区 bcl－ 2蛋白表达进一步增加。结论 bcl－ 2基因在缺血性脑损伤中起保护作用， CGRP的神经保护作用可能与其上调 bcl－ 2蛋白表达有关。
Objective To observe the expression of bcl－ 2 protein and the effect of calcitonin gene related peptide(CGRP)on it in brain tissue during whole cerebral ischemia/reperfusion in rats.Methods The model of whole cerebral ischemia/reperfusion was established by method of carotid negative pressure shunt in rats.Immunohistochemistry method was used to detect the expression of bcl－ 2 in cortex and hippocampal in control group,whole cerebral ischemia 30 minutes/reperfusion 3 hours group(I/R 3h)、 6 hours group(I/R6h),I/R3h plus injecting CGRP into carotid group(I/R3h＋ C)and I/R6h plus injecting CGRP into carotid group(I/R6h＋ C). Results bcl－ 2 protein expression increased significantly in cortex and hippocampal in I/R3h and I/R6h and further in I/R3h＋ C and I/R6h＋ C. Conclusion Bcl－ 2 gene might play a protective role in ischemia/reperfusion brain injury.The protective effect of CGRP might be related to its upregulating effect on bcl－ 2 protein.
出处
《现代康复》
CSCD
2001年第2期54-55,共2页
Modern Rehabilitation
基金
辽宁省自然科学基金资助项目! (619019)
