摘要
以大菱鲆(Scophthalmus maximus)为实验材料,研究其繁殖期内卵子和卵巢液中磷酸酶活性变化规律及其与受精率相关性。结果表明,在大菱鲆繁殖周期内,卵子碱性磷酸酶和酸性磷酸酶活性呈相反的变化趋势,且排卵中期卵子碱性磷酸酶和酸性磷酸酶活性分别显著低于和高于早期和晚期(P<0.05),而卵巢液中碱性磷酸酶和酸性磷酸酶活性呈同卵子中相反的变化趋势;此外卵子碱性磷酸酶和酸性磷酸酶及卵巢液酸性磷酸酶活性同受精率显著相关(P<0.05)。以上结果表明,大菱鲆卵子和卵巢液中碱性磷酸酶和酸性磷酸酶活性同受精率存在着显著相关性,其活性变化在一定程度上反映卵子质量优劣,可作为评判卵子质量辅助指标。
We investigated the activity and variation of phosphatase in eggs and ovarian fluids during the reproductive cycle of turbot(Scophthalmus maximusL.),and explored whether and how phosphatase activity is influenced by fertilization rate.Results of experiments demonstrate that the activity and variation of the alkaline phosphatase and acid phosphatase in eggs was opposite in general to those in ovarian fluid.In eggs,alkaline phosphatase activity in ovulated metaphase was significantly lower than those of the early and late stage of ovulation.In contrast,acid phosphatase activity was strikingly higher than those of the early and late stage of ovulation.In addition,we found that the alkaline phosphatase activity in both eggs and ovarian fluid were closely related tofertilization rate,and acid phosphatase activity in ovarian fluid was much affected by fertilization rate.Therefore,activities of both alkaline and acid phosphatases in eggs and acid phosphatase activity in ovarian fluid were significantly related to fertilization rate,which may be applied to indicate the quality of turbot eggs.
作者
贾玉东
孟振
刘新富
高淳仁
牛华欣
雷霁霖
MENG Zhen;LIU Xin-Fu;GAO Chun-Ren;NIU Hua-Xin;LEI Ji-Lin;JIA Yu-Dong(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao Key Laboratory forMarine Fish Breeding and Biotechnology,Qingdao,266071)
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2013年第6期1530-1535,共6页
Oceanologia Et Limnologia Sinica
基金
国家鲆鲽类产业技术体系建设高效养殖模式专项资金项目,CRAS-50号
国家自然科学基金,31302205号
中国博士后基金,2012M511559号,2013T60690号
山东省自然科学基金,ZR2012CQ024号
山东省优秀中青年科学家基金,BS2013SW004号
关键词
大菱鲆
卵子
卵巢液
碱性磷酸酶
酸性磷酸酶
受精率
turbot Scophthalmus maximus
egg
ovary fluids
alkaline phosphatase
acid phosphatase
fer-tilization rate
