摘要
目的:探讨以清化湿热瘀毒法组方的紫七软肝方含药血清对巨噬细胞条件培养液诱导大鼠肝星状细胞(HSC-T6)活化增殖的影响。方法:体外应用巨噬细胞条件培养液培养诱导HSC-T6活化,在2.2、1.1、0.55 g·ml-1质量浓度的紫七软肝方含药血清分别对其作用24h、48h和72h后,MTT法检测其对HSC-T6增殖的影响。结果:紫七软肝方含药血清对活化后HSC-T6具有显著的抑制作用(P<0.01),且优于黄芪一贯煎方组,高浓度紫七软肝方组的抑制作用优于秋水仙碱组,中、低浓度组不及秋水仙碱组。结论:肝纤维化病程中"湿热瘀毒"为疾病病理因素,根据"清化湿热瘀毒法"拟制的紫七软肝方对肝星状细胞的活化增殖有一定抑制作用,可能是其抗肝纤维化的作用机制之一。。
Objective： To the action of serum containing Ziqi Ruangan decoction in the activated Hepatic Stellate Cells-T6 （ HSC-T6 ） strains cuhured by macrophage conditioned medium in vitro. Methods： When the mass concentration of serum contai- ning Ziqi Ruangan decoction was 2. 2g, 1. lg and 0. 55 g·ml^-1, the activated HSC-T6 was cultured on 24, 48 and 72 hours re- spectively. The anti-proliferative acpacity was detected used by MTT assay. Results： Serum containing Ziqi Ruangan decoction inhibited the proliferation of activated HSC-T6 in a dose-dependent manner （P 〈 0. 01 ） . The curative effect on hepatic fibrosis of serum containing Ziqi Ruangan decoction was better than Huangqi Yiguan decotion. Only high concentration group was better than the positive control group, and others was not as good as the latter. Conclusion： ＂purifying damp, heat, blood stasis and toxin＂ conduct as the pathogenesis in hepatic fibrosis. Serum containing Ziqi Ruangan decoction, based on the theory of ＂purif- ying damp, heat, blood stasis and toxin＂, can inhibit the proliferation of ouivated HSC-T6, it is one of the machenism of trent hepatic fibrosis.
出处
《中西医结合肝病杂志》
CAS
2014年第1期37-40,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
江苏高校优势学科建设工程基金资助项目(No.YS2012ZYX109)
江苏科技厅基础研究计划基金资助项目(No.BK2010563)
关键词
紫七软肝方
湿热瘀毒
肝纤维化
肝星状细胞
MTT法
Ziqi Ruangan decoction
damp
heat, blood stasis
toxin
hepatic fibrosis
hepatic stellate cells-T6strains
MTY assay
