摘要
用不同浓度的T-2毒素染毒Sertoli细胞24h。染毒结束后采用MTT法检测细胞相对活力,常规方法检测SOD、过氧化氢酶(CAT)和谷胱肽过氧化物酶(GSH-Px)活性以及丙二醛(MDA)含量,彗星试验检测DNA损伤。结果显示,与对照组比较,随着T-2毒素染毒剂量的增加,Sertoli细胞的超氧化物歧化酶(SOD)、CAT和GSH-Px活性显著下降(P<0.05),MDA含量显著上升(P<0.05),DNA的损伤程度则加重的极为显著(P<0.01)。结果表明,T-2毒素可显著抑制Sertoli细胞活力,并通过氧化应激损伤细胞的DNA。
Sertoli cells were inoculated with T-2 toxins in different concentrations. Twenty-four hours after inoculation,relative viability of cells was detected by MTT method, activities of SOD, CAT and GSH-Px,and MDA content in culture medium of cells were tested by routine methods, and cell DNA damage was observed by comet assay. The activities of SOD, CAT and GSH-Px were decreased significantly （P 〈 0.0S）, the MDA contents were increased significantly （P〈0.05） and the degree of DNA damage was very significantly increased （P〈0. 01） in Sertoli cells with T-2 toxin compared to those without T-2 toxin. These changes were occurred in a dose de- pendent manner. T-2 toxin inhibits viability of Sertoli cell,and induces oxidative stress which con- sequently causes DNA damage in Sertoli cell.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2014年第3期481-484,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31302157)
