摘要
目的:通过检测肺癌组织、良性病变组织和对照组织中CDH13基因及其甲基化水平,结合临床病理资料,找出其与肺癌发生、发展的关系。方法:采用逆转录聚合酶链反应(RT-PCR)法和特异性甲基化PCR(MSP)法。结果:肺癌组CDH13基因甲基化阳性率为46.67%(21/45),对照组为2.22%(1/45),良性病变组为6.67%(3/45),前组与后两组之间的差异均有统计学意义(P<0.05)。CDH13基因在肺癌中为0.5273±0.0687,在对照组中为0.8345±0.0385、在良性病变组织为0.8632±0.0465,前组与后两组之间的差异均有统计学意义(P<0.05)。Ⅲ期肺癌组织的CDH13基因甲基化阳性率61.90%(13/21)明显高于Ⅰ+Ⅱ期33.33%(8/24)(P<0.05)。有淋巴结转移的肺癌组织中CDH13基因甲基化阳性率70.59%(12/17)与无转移组的32.14%(9/28)差异有统计学意义(P<0.05)。肺癌组织中CDH13基因甲基化阳性组的T-钙黏蛋白阳性率23.81%(5/21)显著低于甲基化阴性组54.17%(13/24)(P<0.05)。结论:肺癌组织中T-钙黏蛋白基因含量明显降低,存在着CDH13基因启动子5'CpG岛高甲基化,且CDH13基因的高甲基化水平随着肺癌临床分期和淋巴结转移而增高。
Objective：To find out the relationship with the occurrence and development of lung cancer by detecting the level of CDH13 and the rate of methylated CDH13 in lung cancer tissues, benign lesions and con- trol group combined with clinical pathology data. Method：We tested CDH13 and its methylation index by RT-- PCR method and specificity of methylation Polymerase Chain Reaction （MSP）. Results：The positive rate of methylated CDH13 is 46. 67% （21/45） among patients with lung cancer, and 2.22% （1/45） among patients in control group; and 6.67% （3/45） in benign lesions. The differences between the former group and the lat- ter two groups have statistical significance （P 〈 0. 05）. CDH13 is 0. 5273 ±0. 0687 in lung cancer group 0. 8345 ＋_0. 0385 in control group; 0. 8632± 0. 0465 in benign lesions. The differences between the former group and the latter two groups have statistical significance （P 〈 0.05）. The positive rate of methylated CDH13 in g stage Lung cancer tissue（61.90%,13/21）is apparently higher than that in I ＋ Ⅱ stage Lung cancer tissue （33.33%8/24） （P 〈 0.05）. The positive rate of methylated CDH13 in lung cancer tissues （70. 59 %, 12/17）with lymph node metastasis is apparently higher than that in lung cancer tissues （32.14%, 9/28） （P 〈 0.05） with no lymph node metastasis. The CDH13 positive rate of Methylation positive group （23.81%, 5 / 21 ） was found to be significantly lower than that in Methylation negative group （54.170/g, 13/24） in lung cancer tissue （P 〈 0.05）. Conclusion：The truncated--cadherin is significantly lower in lung cancer tis- sues. There are high CDH13 gene promoter 5 ＇CpG island methylation in lung cancer tissues. The level of high CDH13 gene methylation is increased along with the lung cancer and lymph node metastasis and clinical stage.
出处
《黑龙江医药科学》
2014年第1期34-35,共2页
Heilongjiang Medicine and Pharmacy
基金
黑龙江省卫生厅科研课题项目
编号:2011-36
