摘要
目的 研究实验动物化脓性心包炎心肌损害与氧自由基、谷胱甘肽、维生素E之间的关系。方法 家兔 2 4只 ,分为 2组 ,各 12只。实验组 :心包腔内注入 10 9个·ml-1·kg-1青霉素敏感的金葡菌溶液。对照组 :心包腔内注入等量生理盐水 (1ml/kg)。注射前、后测定血浆中丙二醛(MDA)、谷胱甘肽 (GSH)与维生素E(VitE)含量。注射后 2 1d全部动物处死。取心肌病理检查、AT Pase活性与电镜检查 ,同时测定心肌匀浆中的MDA与GSH。结果 注射后的血浆中MDA值高于注射前与对照组 (P <0 .0 5 ) ;而血浆中的GSH与VitE的测定值则低于注射前与对照组 (P <0 .0 5 )。动物处死后的心肌匀浆中的MDA值高于注射前与对照组 (P <0 .0 1) ;而GSH值则低于注射前与对照组 (P <0 .0 1)。结论 化脓性心包炎时氧自由基增多 。
Objective To search for the myocardiac damage in relation to malondialdehyde (MDA), glutathione (GSH) and Vitamine E ( Vit E) in pyogenic pericarditis.Methods Twenty four rabbits were divided equally into two groups. The pericardial cavity of Group I, II was injected into staphylococcus aureas (10 9/ml) and normal saline (control group) respectively. The levels of MDA, GSH and Vit E were measured before and after the injection. All rabbits were killed on the 21st day after injection. The pathological changes and ultrastructure were observed, and the levels of MDA, GSH and Vit E in serum were determined. The myocardial concentration of MDA and GSH were also measured.Results As compared with pre injection and that in the Group Ⅱ, the levels of MDA in serum were increased ( P < 0.05 ), while the levels of GSH and Vit E were decreased ( P < 0.05 ). The myocardiac damage were observed under light microscope and electron microscope.Conclusion The increased free oxygen radical , and decreased GDH and Vit E are important factors leading to myocardiac damage in pyogenic pericarditis.
出处
《中华小儿外科杂志》
CSCD
北大核心
2001年第1期45-47,共3页
Chinese Journal of Pediatric Surgery
