摘要
利用固定波长荧光分光光度（fixed-wavelength fluorescence,FF）法测定了太湖周边罗非鱼（Oreochromis niloticus）和洪泽湖周边鲫鱼（Carassius auratus）胆汁中多环芳烃（polycyclic aromatic hydrocarbons,PAHs）的代谢物水平,其目的是通过内滤效应和变异分析来优化该方法的稀释倍数和最佳样本量,并进一步判断PAHs的源解析.结果显示,鲫鱼和罗非鱼各环PAHs荧光比值与胆汁稀释梯度之间并未表现出显著相关性,揭示内滤效应并不存在;但是,各荧光比值随着稀释倍数的增加而呈现明显上升趋势,当稀释倍数逐步增加时,各环PAHs荧光值的变异也在增加,而较大的数据变异则导致功效值降低和样本量增加,因此将稀释倍数控制在1∶1000左右较为合适,此时鲫鱼和罗非鱼的最佳样本量分别为6～7和27～62;太湖和洪泽湖养殖场周围水体中PAHs的污染均为石油源,其原因可能是长江下游工业发达、行船漏油现象频繁所致.该研究可为合理利用FF法对PAHs进行生物监测提供参考.
Levels of polycyclic aromatic hydrocarbons （PAHs） metabolites in biles of Nile tilapia （ Oreochromis niloticus） from aquafarms around the Taihu Lake and of crucian carp （ Carassius auratus） from aquafarms around the Hongze Lake were determined with the fixed wavelength fluorescence method （FF method） to explore for appropriate dilution rate and optimum sample size to optimize this determination method through inner filter effect and variation analysis, and further to perform source apportionment of the PAHs. Results show no significant correlation between fluorescence ratio of the PAHs in biles of the crucian carp and Nile tilapia and biliverdin, indicating that there was no inner filter effect existing in bile; however, the fluorescence ratios of various PAHs all displayed obvious rising trends with increasing dilution rate and the variation between the various PAHs also intensified. Intensified variation would lead to lower effect value and bigger sample size. It is, therefore, proper to control the dilution rate at around 1 ： 1 000, and correspondingly, the optimum sample size of crucian carp and Nile tilapia would be 6-7 and 27-62, respectively. The PAHs pollutants in the water body around the aquafarms in the two lake regions are all petroleum-sourced, which is possibly the result of fuel leakage of vessels sailing in the lower reaches of the Yangtze River and of the waste discharge from the developing industry in the regions. The findings of this research may provide some reference for rational use of FF method in bio-testing PAHs.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2014年第1期107-112,共6页
Journal of Ecology and Rural Environment
基金
中央级公益性科研院所基本科研业务费专项(2011JBFA16)
国家罗非鱼产业技术体系(CARS-49)
关键词
固定波长荧光分光光度法
多环芳烃
内滤效应
功效分析
源解析
fixed wavelength fluorescence
polycyclic aromatic hydrocarbons
inner filter effect
effect analysis
source apportionment
