摘要
目的 :探讨肿瘤坏死因子 - α(TNF- α)在中耳胆脂瘤中的表达及其对邻近骨质的作用。方法 :应用TNF- α单克隆抗体对 18例中耳胆脂瘤组织和 8例正常外耳道、面部皮肤和鼓膜进行免疫定位检测。结果 :TNF-α定位于胆脂瘤组织的上皮及上皮下结缔组织 ,较正常外耳道及鼓膜的染色明显增强。结论 :TNF- α在中耳胆脂瘤组织中有较高表达并通过两条途径引起骨质吸收 :1TNF- α作为自分泌调节因子引起破骨性骨吸收 ;2 TNF-α作为中间信使 ,激活炎性细胞释放一系列生物酶引起骨组织脱钙 ,骨基质、骨蛋白溶解 ,最终导致骨质吸收。
Objective:To determine the expression and localization of tumor necrosis factor alpha(TNF α) in human middle ear cholesteatoma and its effects on bone resorption.Method:The distribution of TNF α in 18 cholesteatoma tissues and 8 normal external ear canal skin, facial skin and tympanic memebrane were studied immunohistochemically with paraffin embedded sections.Result:TNF α was localized in the epitheliun and connective tissue of cholesteatomas studied, particularly on basal and spinous cells and macrophages.The epithelium of normal external ear canal skin , facial skin and tympanic membrane was slightly stained.Conclusion:This study revealed significantly increased level of TNF α in cholesteatoma tissues.TNF α acts both directly,by causing bone erosion as an autocrine growth factor,and indirectly as an important mediator by stimulating the release of enzymes causing bone destruction.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2001年第2期66-67,I002,共3页
Journal of Clinical Otorhinolaryngology
关键词
中耳胆脂瘤
肿瘤坏死因子-A
骨质吸收
Middle ear cholesteatoma
Tumor necrosis factor alpha
Bone destruction
