摘要
采用PCR-RFLP方法对玉山黑猪的ESR基因和FSH-β基因多态性进行检测,并分析其与繁殖性能间的关系。结果表明,ESR基因的3种基因型AA、AB、BB在玉山黑猪中的频率分别为0.417、0.300、0.017,各基因型对应的产仔数存在差异,总体表现BB>AB>AA,其中初产仔猪数BB型多于AA型1.21头,多于AB型0.91头;BB型经产仔猪数也高于AB型和AA型,但3种基因型之间差异不显著。FSH-β基因的3种基因型AA、AB、BB在玉山黑猪中的频率分别为0.567、0.333、0.100,各基因型对应的产仔数存在差异,总体表现BB>AB>AA,初产仔猪数BB型多于AA型0.80头(P<0.05),多于AB型0.43头(P>0.05);BB型的经产仔猪数也是3种基因型中最高的,但彼此之间差异不显著。ESR和FSH-β基因在初产仔猪数及经产仔猪数上都具有显著的加性遗传效应(P<0.05)。
In this study , PCR-RFLP method was used to detect the polymorphisms of genes ESR and FSH-βin Yushan black pigs , and the relationship between the genic polymorphism and reproductive performance was analyzed .The results indicated that the genotypes AA , AB and BB of ESR gene in Yushan black pigs had the frequency of 0.417, 0.300 and 0.017 respectively;the litter size of various genotypes was different , and generally revealed the following sequence：BB＆gt;AB＆gt;AA.The number of pri-miparous piglets of genotype BB was 1.21 heads more than that of genotype AA , and 0.91 heads more than that of genotype AB . The number of multiparous piglets of BB was also more than that of AB and AA , but there were no significant differences in this a-mong 3 genotypes.The frequency of genotypes AA , AB and BB of FSH-βgene in Yushan black pigs was 0.567, 0.333 and 0. 100 respectively;there were differences in litter size among 3 genotypes, showing the trend of BB ＆gt;AB＆gt;AA in general.The num-ber of primiparous piglets of BB was 0.80 heads more than that of AA （P＆lt;0.05）, and 0.43 heads more than that of AB （P＆gt;0. 05）.The genotype BB also had the highest multiparous piglets , but there were no significant differences among 3 genotypes.Both ESR gene and FSH-βgene had obvious additive genetic effect in the number of primiparous piglets and number of multiparous pig -lets （P＆lt;0.05）.
出处
《江西农业学报》
CAS
2014年第3期107-109,共3页
Acta Agriculturae Jiangxi
基金
国家农业科技成果转化资金项目"优质高效玉山黑猪新品系选育中试与示范"(2012G B2C 500226)
