摘要
钒（V）对牛血红蛋白模拟酶催化过氧化氢氧化罗丹明B体系有强烈的抑制作用,据此建立了酶催化分光光度法测定痕量钒（V）的新方法.试验从pH值、罗丹明B浓度、过氧化氢浓度、牛血红蛋白浓度和反应时间等方面探讨了体系的实验条件.在pH 9.8的NH3·H2O-NH4Cl缓冲溶液中,当罗丹明B、H2O2和血红蛋白的浓度分别为1.5×10-5 mol/L、1.5×10-4 mol/L和2.0×10-6 mol/L时,测定钒（V）的线性范围为3.1×10-4～6.2×10-3 g/L,方法的检出限为2.1×10-6 g/L.1 000倍Na＋、K＋、Cl-、NO3-、NH4＋、F、BrO3-,500倍Ca2＋、Fe3＋、Al3＋、Mn2＋、Ba2＋、CO32-,100倍C2O42-,5倍Cu2＋对钒（V）的测定没有干扰.对环境水样中痕量钒（V）进行测定,结果的相对标准偏差（n=7）为3.7％,与萃取分光光度法的结果基本一致,t检验结果表明两种方法没有显著性差异.
Vanadium（V） had strong quenching effect on the catalytic spetrophotimetric system of hemoglobin analog enzyme.Based on this,a novel enzymatic spectrophotometry for determining vanadium（V） was established.The influence of acidity,rhodamine B concentration,H2O2 concentration,hemoglobin concentration and reaction time on the system was studied.In NH3 · H2O-NH4Cl buffer solution of pH 9.8,when the concentration of rhodamine B,H2 O2 and hemoglobin were 1.5 × 10 5 mol/L,1.5 ×10-4 mol/L and 2.0 × 10-6 mol/L respectively,the linear range for the determination of vanadium（V） was 3.1×10 4-6.2×10-3g/L with the detection limit of 2.1×10-6g/L.1 000 times of Na＋,K＋,Cl,NO3-,NH4 ＋,F and BrO3-,500 times of Ca2＋,Fe3＋,Al3＋,Mn2＋,Ba2＋ and CO32-,100times of C2O42-,5 times of Cu2＋ did not interfere the determination of vanadium（V）.The RSD was 3.7 ％ for 7 parallel determinations of vanadium（V） in environmental water,The results obtained by the extraction spectrophotometry and the proposed method are statistically compared.The t-test showed that the two methods had no significant differences.
出处
《冶金分析》
CAS
CSCD
北大核心
2014年第1期59-62,共4页
Metallurgical Analysis
基金
河南省基础与前沿技术研究计划资助项目(132300410176)
河南省教育厅科学技术研究重点资助项目(14A150015)
