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硫化氢对小鼠背根神经节河豚毒素敏感型钠电流的影响 被引量：1

Effects of H 2 S on tetrodotoxin-sensitive sodium currents in mouse dorsal root ganglion neurons
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摘要 研究硫化氢(H2S)对小鼠背根神经节(DRG)细胞河豚毒素敏感型(TTX-S)钠电流的影响。急性分离小鼠DRG细胞,应用全细胞膜片钳技术,观察H2S供体NaHS对TTX-S钠电流的影响。结果显示,NaHS浓度依赖性地增加TTX-S钠电流,其半数最大激活浓度(EC50)为119μmol/L,Hill系数为1.105;100μmol/L NaHS可使TTX-S钠电流激活曲线向去极化移动约9.8 mV,稳态失活曲线向去级化方向移动约10.4 mV,但对恢复曲线无明显影响。因此,H2S能增加TTX-S钠通道电流,并改变TTX-S钠通道稳态激活和失活动力学特征,这可能是H2S参与疼痛发生发展的机制之一。 The effects of hydrogen sulfide（H2S）on tetrodotoxin-sensitive（TTX-S）sodium currents in mouse dorsal root ganglion（DRG）neurons, were studied by use of the whole-cell patch clamp technique. NaHS（a donor of H2S）increased TTX-S sodium currents in a concentration-dependent manner; the EC50 was 119 μmol/L and Hill coefficient was 1. 105. NaHS 100 μmol/L markedly shifted the steady state activation and inactivation curves of TTX-S sodium currents towards more positive potential to 9. 8 and 10. 4 mV, respectively, yet with no significant difference on recovery from inactivation of TTX-S sodium channels. Therefore, H2S increased the TTX-S sodium currents and altered the activation and the inactivation kinetics of TTX-S sodium channel, which may contribute to one of its mechanisms in pain.
作者 鲜文颖 徐鲁杰 张广钦
机构地区 中国药科大学临床药学教研室
出处 《中国药科大学学报》 CAS CSCD 北大核心 2014年第1期97-101,共5页 Journal of China Pharmaceutical University
关键词 硫化氢 背根神经节 TTX敏感型钠电流 hydrogen sulfide dorsal root ganglion tetrodotoxin-sensitive sodium current
分类号 R965 [医药卫生—药理学]
  • 相关文献

参考文献14

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二级参考文献7

共引文献3

同被引文献10

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中国药科大学学报
2014年 第1期

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