摘要
目的:观察小陷胸汤对动脉粥样硬化大鼠主动脉斑块面积及NF-κB表达的影响,探讨其抗动脉粥样硬化的机制。方法:健康雄性Wistar大鼠60只,随机分为对照组、模型组、阿托伐他汀组(0.005 g·kg-1)及小陷胸汤低(2.1 g·kg-1)、中(4.2g·kg-1)、高(8.4 g·kg-1)剂量组。对照组喂普通饲料,其余各组采用高脂饲料联合一次性腹腔注射VD360万IU·kg-1造模。第3周开始各治疗组灌胃给药,对照组与模型组灌服等容积生理盐水,共灌胃12周。末次给药12 h后,处死大鼠,测量主动脉斑块面积,Western blot法检测NF-κB的表达。结果:与模型组相比,小陷胸汤低(2.1 g·kg-1)、中(4.2 g·kg-1)、高(8.4 g·kg-1)剂量组NF-κB的表达明显降低(P<0.05),小陷胸汤中(4.2 g·kg-1)、高(8.4 g·kg-1)剂量组斑块面积较模型组显著减少(P<0.01或P<0.05),而低剂量组(2.1 g·kg-1)斑块面积无明显变化(P>0.05)。结论:小陷胸汤在一定程度上可抑制动脉粥样硬化大鼠主动脉斑块的形成及其NF-κB的表达,这可能是小陷胸汤抗AS作用机制之一。
OBJECTIVE To observe the effects of Xiaoxianxiong decoction on plaque area and NF-κB expression in aorta of atherosclerotic rats. METHODS 60 healthy male Wistar rats were randomly divided into control group, atheroseerosis model group, low（2. 1 g. kg- 1 ）, medium（4. 2 g. kg-1 ） and high（8. 4 g kg- 1 ） dose of Xiaoxianxiong decoction group, and atorvastatin group （0. 005 g.kg-1 ）. The control group was fed with normal diet, the rest of the groups were fed with high fat diet and injec- ted Vm 60 million IU.kg i in the abdomen by once. At the third week, each treatment group began to give the drugs by ga- vage, simultaneously the control and the model group were fed with volume of normal saline, 12 weeks in total. 12h after the last administration, all rats were sacrificed, measured plaque area and NF-κB expression. RESULTS Compared with the model group, NF-κB expression in low（2. 1 g kg- 1 ）, medium （4. 2 g kg-1 ） and high （8. 4 g kg- 1 ） dose of Xiaoxianxiong decoction group was lower（P〈0. 05）, plaque area in medium（4. 2 g.kg-l ） and high（8. 4 g.kg-1 ） dose of Xiaoxianxiong decoction group was significantly reduced（ P〈0. 01 or P〈0. 05）, while there was no obvious change in low-dose（2. 1 g-kg-1 ） group（P〉 0. 05）. CONCLUSION Xiaoxianxiong decoction, to some extent, can reduce NF-κB expression,inhibiting the plaque formation. This may be one of the mechanisms of anti-AS action of Xiaoxianxiong decoction. KEY WORDS：
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2014年第4期284-286,共3页
Chinese Journal of Hospital Pharmacy
基金
湖北省卫生厅中医药中西医结合科研项目(编号:2012Z-Y33)
