摘要
目的通过观察腺苷预处理后大鼠局灶性脑缺血再灌注区脑组织的病理结构、脑梗死面积及血管内皮生长因子(VEGF)的表达,探讨腺苷预处理诱导脑缺血耐受的可能作用机制。方法制作大鼠脑缺血再灌注损伤模型。60只SD大鼠随机分为3组:假手术组(F组)、缺血再灌注组(IR组)、腺苷预处理组(AP组),再按缺血再灌注后不同时间把各组随机分成4个亚组(每亚组5只大鼠)。通过TTC染色观察脑梗死体积,并应用免疫组化法检测各组大鼠脑组织VEGF的表达。结果 (1)TTC染色正常脑组织呈鲜红色,梗死区脑组织呈苍白色,并且随着再灌注时间的延长可见梗死区扩大,腺苷预处理组的脑梗死体积小于缺血再灌注组。(2)F组可见少量VEGF阳性表达,IR组和AP组在脑缺血再灌注后2 h开始出现VEGF阳性表达的细胞数量增多,24 h达到高峰,72 h下降,AP组在6 h、24 h VEGF阳性表达比IR组增强(P均<0.05),在72 h时AP组VEGF阳性表达较IR组减少(P<0.05)。结论腺苷预处理能够进一步增强大鼠局灶性脑缺血再灌注损伤后VEGF的表达;VEGF的表达增加可能是腺苷预处理诱导脑缺血耐受和产生神经保护作用的分子机制之一。
Objective To explore molecular mechanisms behind cerebral ischemia tolerance resulted by adenosine pretreatment through observing the pathogenic structure, infarct size and vascular endothelial growth factor （VEGF） expres- sion in rat brain tissues which went through ischemia reperfusion with or without adenosine pretreatment. Methods To es- tablish the rat model ,60 SD rats were divided into 3 groups ：the sham-operated group （F group） , the ischemia reperfusion group （IR group） and the adenosine pretreated group （AP group）. And each group was divided into 4 subtypes according to different reperfusion time （ each subtype containing 5 rats）, TFC staining was used to label infarct area and immunohisto- chemistry to detect VEGF expression. Result Normal brain tissue exerts bright red while infarct tissue pale white after TTC staining. Even though infarct part expanded along with reperfusion time, they were smaller in AP group than in IR group. Lit- tle VEGF expression was detected in F group. In IR and AP group, VEGF expression emerged at the point of 2 h after reper- fusion, peaked at 24 h and declined at 72 h. At 6 h, VEGF expression in AP group was higher than that in IR group but de- creased quickly so that in 72 h the VEGF expression in AP group was lower than that in IR group. Conclusion Adenosine pretreatment enhanced VEGF expression in the infarct area of rat brain tissue after ischemia reperfusion,which is likely to participate in the protective role of adenosine in ischemia reperfusion.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2014年第2期107-110,共4页
Journal of Apoplexy and Nervous Diseases
关键词
腺苷预处理
脑缺血再灌注损伤
血管内皮生长因子
缺血耐受
Ademosine preconditioning
Focal cerebral ischemia- reperfusion
Vascular endothelial growth fac-tor
Ischemic tolerance
