摘要
目的探讨构建新西兰兔脊柱结核模型的实验方法，并评价此种方法的可行性。方法62只新西兰兔随机分为致敏造模组（经弗氏完全佐剂致敏后造模）20只、未致敏造模组（造模前未经弗氏完全佐剂致敏）20只、对照组20只、空白组2只。致敏造模组兔经弗氏完全佐剂致敏后，于L4上终板下方钻孔，填人明胶海绵，在其中浸注种植H37Rv人型标准结核杆菌混悬液0．1ml；未致敏造模组未经弗氏完全佐剂致敏，浸注H37Rv人型标准结核杆菌混悬液0．1ml；对照组浸注液体培养基与生理盐水混悬液0．1ml；空白组不做任何处理。结果术后8周兔存活率致敏造模组为89．5％、未致敏造模组60．0％，对照组94．7％、空白组100％；致敏造模组成活兔造模全部成功。致敏造模组造模椎体破坏情况：术后4周x线片为50％、重建CT和MRI均为83．3％；术后8周X线片为58．8％、CT重建和MRI均为100％。术后8周，肉眼观察全部成活兔造模椎体均出现骨破坏、肉芽及坏死组织形成，腰大肌脓肿发生率为17．6％；组织病理学显示造模椎体均有大量淋巴细胞和类上皮样细胞、骨小梁断裂、凝固性坏死物质；细菌培养阳性率为52．9％。未致敏造模组术后8周有75％成活兔造模椎体出现骨破坏，腰大肌脓肿发生率为100％。对照组与空白组无上述改变。结论经弗氏完全佐剂致敏后，在新西兰兔腰椎椎体的上终板下钻孔、明胶海绵填塞、种植H37Rv结核杆菌标准菌株的方法，可成功构建兔脊柱结核模型。
Objective To establish and evaluate a rabbit model of spinal tuberculosis. Methods Sixty-two New Zea- land rabbits were randomly divided into 4 groups： experimental A group （sensitized by complete Freund＇s adjuvant, n=20）, experi- mental B group （not sensitized by complete Freund＇s adjuvant, n=20）, control group （n=20） and blank group （n=2）. After experi- mental A group was sensitized by complete Freund＇s adjuvant, a hole was drilled beneath the upper endplate of the IA vertebral body and filled with a gelfoam sponge, which was infused with 0.1 ml H37Rv standard Mycobacterium tuberculosis suspension. Ex- perimental B group was filled with a gelfoam sponge infused with O. 1 ml H37Rv standard Mycobacterium tuberculosis suspension （culture medium and saline in control group）. The blank group received no treatment. Results The survival rate of postoperative 8 w was 89.5%, 60.0%, 94.7% and 100% in the experimental A, experimental B, control and blank group respectively. The model was successfully established in all surviving rabbits of the experimental A group. The vertebral body destruction of the experimen- tal A group was 50% by X-ray and 83.3% by CT reconstruction and MRI at 4 w, while it was 58.8% by X-ray and 100% by CT re- construction and MRI at 8 w. All surviving rabbits of the experimental A group had vertebral destruction, granulation and necrosis while 17.6% had psoas abscess at 8 w. Histopathology study revealed numerous lymphocytes and epithelioid cells, trabecular bone fracture and coagulative necrosis in the vertebrae of the experimental A group. The bacterium culture was 52.9% positive. There were 75% vertebrae damage and 100% psoas abscess in experimental B group. Nither control nor blank group showed such chang- es. Conclusion A New Zealand rabbit of spinal tuberculosis model can be successfully established by drilling a hole in the up- per endplate of the vertebral body, filling with gelfoam sponge infused with H37Rv standard Mycobacterium tuberculosis suspen- sion after sensitized by complete Freund＇s adjuvant.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2014年第2期216-223,共8页
Chinese Journal of Orthopaedics
基金
国家自然科学基金（81060149,81260282）
宁夏自然科学基金（NZ11275）
关键词
结核
脊柱
模型
动物
结核分枝杆菌
Freund佐剂
兔
Tuberculosis, Spinal
Models, Animal
Mycobacterium tuberculosis
Freund＇s adjuvant
Rabbits
