摘要
以厚皮甜瓜（Cucumismelo）‘W1998’（纯雌系）与‘TopMark’（雄全同株）配制杂交组合获得的RIL6群体为材料，对分离后代雌雄异花同株，雄全同株，完全花植株及纯雌株进行转录组测序，利用生物信息学分析方法，获得花器官发育相关基因序列Un16008，命名为CmTs2。该序列位于甜瓜第12条染色体上，长度1842bp，119-832之间存在一个包含237个氨基酸的最大开放式阅读框，成熟蛋白分子量为25358．8D，该蛋白不稳定系数为21．6，脂肪系数是99．11，平均亲水系数为0．078。通过Blast比对发现该基因与多种作物Tasselseeds2（TS2）基因具有高度同源性，其中与黄瓜Tasselseeds2基因同源性高达96％。通过实时荧光定量PCR检测，发现该基因在甜瓜雌雄异花同株花和叶片中表达量均高于其它性别类型植株，在纯雌株中表达量最低，说明该基因可能参与甜瓜花器官发育，尤其是雌花发育的过程。
Using transcriptom sequencing technology to analysis and detected different expressed gene among monoecious, andromonecious, gynoecious and hermaphrodite families from WI998 （gynoecious） cross Topmark （andromonecious）, a full length of 1 842 bp which locates in chromosome 12, named CmTs2, was obtained from bioinformatics methods. Sequence analysis indicated that the CmTs2 consisting an open reading frame （ORF）which from 119 to 832 nucleotides encoding 237 amino acids withmolecular weight 25 358.8 D, instability index is 21.6 and aliphatic index is 99.11, grand average of hydropathicity is 0.078. Blast alignment analysis indicated highly homologous to TS2 gene especially for cucumber which has 96% homologous relationship, pathway analysis indicated CmTs2 gene located the upstream of gibberellin synthesis pathway. Real time-PCR analysis indicated that the expression level of CmTs2 was highest expressed in andromonecious but lowest in gynoecious in leaf and flower, which showed the CmTs2 gene might involve in the flower development female flower.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第2期349-356,共8页
Acta Horticulturae Sinica
基金
教育部博士点新教师基金项目(20112305120002)
黑龙江八一农垦大学寒地作物实验室开放课题(2012010)
关键词
甜瓜
转录组测序
花器官
基因表达
Cucumis melo
transcriptom sequence
sex expression
gene expression
