摘要
以紫皮大蒜品种‘G075’为试材,研究了鳞茎发育过程中鳞茎外皮花青苷的积累规律及与其生物合成相关的苯丙氨酸解氨酶(PAL)、查儿酮异构酶(CHI)和二氢黄酮醇还原酶(DFR)活性的关系,分析了不同设施栽培方式、基质pH值、基质氮素水平和磷素水平对花青苷生物合成的影响。研究结果表明:随着大蒜鳞茎的发育,鳞茎外皮花青苷含量逐步提高,CHI活性与鳞茎外皮花青苷积累关系密切,其活性变化与花青苷积累趋势吻合;露地栽培温度相对较低的紫皮大蒜花青苷积累高于保护地温度相对较高的栽培大蒜,基质pH 6.5和1/2氮素水平(7.5 mmol·L-1)时对花青苷合成有利,花青苷积累随磷素水平提高呈现先升后降的变化趋势。
Anthocyanin, a water soluble compound is thought to be responsible for the coloration of outer scales of purple garlic. The changes in anthocyanin content in relation to enzymatic activities of phenylalanine ammonia-lyase （PAL）, chalcone isomerase （CHI）, and dihydroflavonol reductase （DFR） were studied in the outer scales of purple garlic ‘G075＇ . The relationships between anthocyanin biosynthesis and environmental conditions （temperature, pH of substrate, supplement of nitrogen and phosphorus）were established. The results show that among these enzymes, the activity of CHI is positively correlated to anthocyanin content in significant level and further postulated as key limiting factor to regulate anthocyanin synthesis in the outer scales of purple garlic. The fluctuation of the CHI activity and anthocyanin content is consistent with that of the environmental conditions. These results obviously demonstrate the environmental conditions play a major role in regulating the enzyme activities of anthocy-anin biosynthesis. Cool temperature in open field, acidic medium （pH 6.5）, low nitrogen level （ 1/2 N： 7.5 mmol. L-1） and optimum phosphorus levels （P- 1 mmol. Ll） are favorable for anthocyanin synthesis in the outer scales of purple garlic.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第2期285-292,共8页
Acta Horticulturae Sinica
基金
国家公益性行业(农业)科研专项(200903018-7)
关键词
紫皮大蒜
鳞茎
外皮
花青苷
相关酶活性
环境条件
purple garlic
outer scales
anthocyanin
activities of related enzymes
environmentcondition
