摘要
以‘丰香’草莓为试材,通过SON-PCR结合RT-PCR技术获得了1个冷诱导转录因子CBF/DREB家族基因编码区全长cDNA序列,命名为FaCBF1,GenBank登录号为FJ767754。该基因包含1个长为636 bp的完整开放阅读框,编码211个氨基酸,预测分子量为23.4 kD,等电点为6.53。氨基酸同源性分析表明,FaCBF1与GenBank中登录的蔷薇科植物CBF/DREB具有较高的同源性。进化树分析表明,草莓FaCBF1与近缘属的月季、苹果和沙梨处于同一进化枝上,而与李属植物进化关系较远。半定量RT-PCR分析显示,FaCBF1能被低温、干旱和高盐胁迫诱导,但对ABA诱导不敏感;在同一诱导时期在根中的表达量高于叶和茎中的表达量。
A CBF/DREB gene named FaCBF1 was cloned from strawberry （Fragaria × ananassa） through SON-PCR and RT-PCR and its GenBank accession number is FJ767754. The FaCBF1 gene from the cDNA contained an open reading frame of 636 bp, which encoded a polypeptide of 211 amino acids residues with a molecular mass of 23.4 kD and a pI of 6.53. The sequence homology comparison showed that the FaCBF1 exhibits a high homology of the deduced amino acids sequences with other Rosaceae CBF/DREB from GenBank. A phylogeny tree showed that the FaCBF 1 had a relatively close evolutionary relationship with Rosa hybrida, Malus ×domestica and Pyrus pyrifolia, and a distant relationship with Prunus plants. Semi-quantitative RT-PCR analysis showed that the expression of FaCBF1 was induced by low temperature, drought, and salt stress, but insensitivity to ABA treatment. The expression level in root was higher than leaf and stem at the same treatment time.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第2期240-248,共9页
Acta Horticulturae Sinica
基金
四川省教育厅重点项目(12ZZ011)
四川农业大学学科建设双支计划项目
关键词
草莓
FaCBF1
基因克隆
基因表达
Fragaria× ananassa
FaCBFI ： gene cloning： gene expression
