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白细胞介素-17对系膜细胞功能的影响及其分子机制 被引量：3

Influence of IL-17 on mesangial cell and its molecular mechanism
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摘要 目的研究白细胞介素(IL)-17对系膜细胞增殖及分泌功能的影响,并探讨其可能的分子机制。方法四甲基偶氮唑盐比色(MTT)法检测IL-17对系膜细胞增殖的影响;酶联免疫法(ELISA)检测IL-17对系膜细胞分泌IL-1β的影响;免疫细胞化学法检测系膜细胞NF-κB的表达。结果不同浓度IL-17在不同时间点对系膜细胞增殖的影响均无统计学意义(P>0.05);IL-17可促进系膜细胞分泌IL-1β,且呈浓度时间依赖性,差异有统计学意义(P<0.05);同时IL-17诱导组NF-κB的表达高于空白对照组,差异有统计学意义(P<0.05)。结论 IL-17可能部分通过NF-κB途径引起系膜细胞分泌炎症因子IL-1β,加重肾脏局部的炎症状态,导致肾脏损伤。 Objectives To investigate the effect of IL-17 on proliferation and secretion of IL-1βin the human mesangial cell line （HMCL） and explore the molecular mechanism. Methods The proliferation of HMCL induced by IL-17 was detected by MTT, the secretion of IL-1βin HMCL was measured by ELISA and the expression of NF-κB was detected by immunocyto-chemistry. Results IL-17 did not impact HMCL proliferation （P＆gt;0.05） but dose-and time-dependently promoted the secretion of IL-1β（P＆lt;0.05）. And the expression of NF-κB induced by IL-17 increased comparing to that in the control group （P＆lt;0.05）. Con-clusions IL-17 may induce HMCL to secrete IL-1βvia NF-κB pathway that results in kidney injury.
作者 王莉 李秋 李翠萃 阳海平 王莉佳 刘玮
机构地区 重庆医科大学附属儿童医院肾脏免疫科 成都市妇女儿童中心医院肾内科 重庆医科大学附属儿童医院儿科研究所
出处 《临床儿科杂志》 CAS CSCD 北大核心 2014年第2期178-182,共5页 Journal of Clinical Pediatrics
基金 国家自然科学基金面上项目(No.81070563) 四川省卫生厅科研项目(No.2012001)
关键词 白细胞介素17 系膜细胞 白细胞介素-1Β interleukine-17 interleukine-1β 转录因子-κB mesengial cell transcription factor-κB
分类号 R363 [医药卫生—病理学]
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参考文献22

  • 1Segerer S, Nelson P J, Schlondorff D. Chemokines, chemo- kine receptors, and renal disease .. from basic science to patho- physiologic and therapeutic studies [J]. J Am Soc Nephrol, 2000, 11(1): 152-176.
  • 2王莉,李秋,王莉佳,李欣.原发性肾病综合征患儿外周血Th17与CD4^+CD25^+Foxp3^+调节性T细胞的水平[J].细胞与分子免疫学杂志,2010,26(8):783-786. 被引量：14
  • 3Steinmetz OM, Turner JE, Paust H J, et al. CXCR3 mediates renal Thl and Thl7 immune response in murine lupus ne- phritis [J]. J Immunol, 2009, 183(7) .. 4693-4704.
  • 4Aggarwal S, Gurney AL. IL- 17: prototype member of an emerging cytokine family [J]. J Leukoc Biol,2002,71 ( 1 ) : 1-8.
  • 5Kawaguchi M, Adachi M, Oda N, et al. IL- 17 cytokine fami- ly [J]. J Allergy Clin Immunol, 2004,114(6) .. 1265-1273.
  • 6Shao XS, Yang XQ, Zhao XD, et al. The prevalence of Thl7 cells and FOXP3 regulate T cells (Treg) in children with pri- mary nephrotic syndrome [J]. Pediatr Nephrol, 2009,24 (9) : 1683-1690.
  • 7Huang J, Siragy HM. Glucose promotes the production of in- terleukine-lbeta and cyclooxygenase-2 in mesangial cells via enhanced (Pro) renin receptor expression [J]. Endocrino- logy, 2009, 150(12): 5557-5565.
  • 8Hong HK, Song CY, Kim BC, et al. ERK contributes to the effects of Smad signaling on oxidized LDL- induced PAI- 1 expression in human mesangial cells [J]. Transl Res, 2006, 148(4): 171-179.
  • 9Sohn M, Tan Y, Klein RL, et al. Evidence for low-density lipoprotein-induced expression of connective tissue growth factor in mesangial cells [J]. Kidney Int, 2005, 67(4): 1286-1296.
  • 10Di Paolo S, Grandaliano G, Gesualdo L, et al. Low- density lipoproteins enhance transforming growth factor-beta 1 (TGF-beta 1) and monocyte chemotactic protein- 1 (MCP- 1) expression induced by cyclosporin in human mesangial ceils [J]. Clin Exp Immunol, 1999, 117(2): 355-360.

二级参考文献12

  • 1Mansour H, Cheval L, Elalouf JM, et al. T-cell transcriptome analysis points up a thymic disorder in idiopathic nephrotic syndrome[ J]. Kidney Int, 2005, 67(6): 2166-2177.
  • 2Wilson NJ, Boniface K, Chan JR, et al. Development, cytokine profile and function of human interleukin 17-producing helper T cells [J]. Nat Immunol, 2007, 8(9) : 950 -957.
  • 3Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, et al. Interleukins 1 beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells [ J ]. Nat Immunol, 2007, 8 (9) : 942 - 949.
  • 4Matsumoto K, Kanmatsuse K. Interleukin-17 stimulates the release of pro-inflammatory cytokines by blood monocytes in patients with IgA nephropathy[ J]. Scand J Urol Nephrol, 2003, 37 (2) : 164 - 171.
  • 5Wang S, Jiang J, Guan Q, et al. Reduction of Foxp3-expressing regulatory T cell infiltrates during the progression of renal allograft rejection in a mouse roodel[ J]. Transplant Immunol, 2008, 19(2) : 93 - 102.
  • 6Cheng X, Yu X, Ding Y,I, et al. The Th17/Treg imbalance in patients with acute coronary syndrome[ J]. Clin Immunol, 2008, 127 (1) : 89 -97.
  • 7Kaneko K, Tuchiya K, Fujinaga S, et al. Th1/Th2 balance in childhood idiopathic nephritic syndrome [ J ]. Clin Nephrol, 2002, 58(6) : 393 -397.
  • 8Stachowski J, Krynicki T, Barth C, et al. Does the changed Th1/ Th2 activity in children with the assessment of body water in children with nephrotic syndrome : initial results [ J ]. Pol Merkur Lekarski, 2000, 8(46) : 222 -224.
  • 9陈杰华,刘恩梅,陈坤华,何云,邓昱,谢晓虹,李欣,王莉佳.哮喘急性发作期患儿外周血Th17水平及其功能初步研究[J].细胞与分子免疫学杂志,2009,25(4):359-361. 被引量：15
  • 10杨军,李成荣,王国兵,黄惠君,祖颖,李永柏.Th17细胞与CD4^+CD25^+调节性T细胞在儿童过敏性紫癜发病机制中的作用[J].临床儿科杂志,2009,27(7):645-648. 被引量：50

共引文献13

同被引文献25

  • 1胡亚美 江载芳.诸福棠实用儿科学[M]第7版[M].北京:人民卫生出版社,2002.1514.
  • 2Shao XS, Yang XQ, Zhao XD, et al. The prevalence of Thl7 cells and FOXP3 regulate T cells(Treg)in chil- dren with primary nephrotic syndrome [J ]. Pediatr Nephrol, 2009,24(9) : 1683-1690.
  • 3Zhang X, J in J, Peng X, et al. Simvastatin inhibits IL-17 secretion by targeting multiple IL-17-regulatory cyto- kines and by inhibiting the expression of IL-17 tran- scription factor RORC in CD4+ imphocytes[J]. J Im- munol, 2008,180(10) : 6988-6996.
  • 4Huh JR, Leung MW, Huang P, et al. Digoxin and its derivatives suppress TH17 cell differentiation by an- tagonizing ROR'/t activity [J]. Nature, 2011, 472 (7344) :486-490.
  • 5Steinmetz OM,Summers SA,Gan PY,et al. The Thl7-de- fining transcription factor RORTt promotes glomerulone- phritis[J]. J Am Soc Nephrol,2011,22(3) :472-483.
  • 6Milner JD. IL-17 producing cells in host defense and atopy [J]. Curt Opin Immunol,2011,23(6) :784-788.
  • 7Gaffen SL. Recent advances in the IL-17 cytokine family [J]. Curr Opin Imrnunol,2011,23(5) :613-619.
  • 8Ohoreschi K, Laurence A, Yang XP, et al. Generation of pathogenic T(H)17 cells in the absence of TGF-13 signal- ling[J]. Nature,2010,467(7318):967-971.
  • 9Wang R, Wan Q, Zhang Y, et al. Emodin suppresses in- terleukin-lbeta induced mesangial cells proliferation and extracellular matrix production via inhibiting P38 MAPK [J]. Life Sci,2007,80(26) 2481-2488.
  • 10Cao B, Ren LL, Zhao F, et al. Viral and Mycoplasma pneumonia community-acquired pneumonia and novel clinical outcome evaluation in ambulatory adult patients in China[J]. Eur J Clin Microbiol Infect Dis, 2010, 29(11): 1443-1448.

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临床儿科杂志
2014年 第2期

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