摘要
目的:观察加减六味地黄汤对多柔比星肾病大鼠的疗效及对内皮素-1(ET-1)和一氧化氮(NO)表达的影响,探讨其临床应用的理论依据方法:40只雄性Wistar大鼠随机分为正常时照组、模型对照组和加减六味地黄汤治疗组。正常对照组(10只)尾静脉一次性注射生理盐水1ml,其余各组(各15只)均采用多柔比星5.5 mg·kg^(-1)一次性尾静脉注射,1周后开始药物干预,治疗组每日灌胃9 g·kg^(-1)加减六味地黄汤,正常对照组和模型对照组灌胃等量生理盐水,持续8周。检测大鼠尿白蛋白/尿肌酐比值,采用放免法及硝酸还原酶法检测外周血清及肾组织匀浆中ET-1和NO的含量。结果:加减六味地黄汤治疗组大鼠较模型对照组,尿白蛋白/尿肌酐比值明显降低(P<0.05),血清及肾组织中ET-1表达含量明显下降(P<0.05),NO表达含量显著升高(P<0.05)。结论:加减六味地黄汤能减少多柔比星肾病大鼠尿蛋白排泄水平,其机制可能与抑制肾组织过度产生ET-1,并能使NO分泌增加有关。
Objective： To investigate the effects of Liuwei Dihuang decoctions on the expression of ET-I and NO in the rats witil nephrosis induced by adriamycin. Methods： Forty male Wistar rats were used. The minimal change nephropathy model was established by the injection of 5.5 mg·kg^-1 adriamycin into the tail vein. After 1 week, the rats were divided into the model group and the Li- uwei Dihuang decoctions intervention group, and the normal control group was established as well. UAlb/UCr was measured. The level of ET-I and NO was measured by radin-immunoassay method and nitrate reductase method, respectively. Results： UAlb/UCr and ET- I in the Liuwei Dihuang decoctions intervention group were decreased significantly compared with those in the control group （ P 〈 0.05 ）. NO was increased significantly in the Liuwei Dihuang decoctions intervention group compared with that in the control group （ P 〈 0.05 ）. Conclusion ： Liuwei Dihuang decoctions can reduce the excretion of urinary protein in rats with nephrosis induced by adria- mycin by suppressing ET-1 overproduction and increasing NO release.
出处
《中国药师》
CAS
2014年第1期14-16,共3页
China Pharmacist
