摘要
以甲基供体S 腺苷酰 L 甲硫氨酸 (SAM )作为分化诱导物 ,处理人急性早幼粒白血病HL 60细胞 ,导致细胞生长受到抑制 ,且表现出一定范围的SAM浓度依赖性和时间依赖性 .在 10μmol·L- 1最适浓度下 ,细胞分化指数 (NBT阳性率 )达到 16% ;中性粒细胞标志酶酸性磷酸酶活性增高 ;细胞出现明显的分化特征性形态变化 ,说明SAM具有诱导HL
S adenosyl L methionine (SAM) is chosen as an inducer for differentiation of HL 60 cells. It is found that the growth of HL 60 cells is inhibited. The ability of NBT reduction of HL 60 cells is also obtained during treatment of SAM. The percentage of NBT positive cells in the fourth day treated by SAM is 16%, which occurred in the presence of 10 μmol·L -1 SAM. There is a positive correlation between the inhibition and concentration of SAM in certain range of SAM concentration. Meanwhile, the activity of acid phosphates, which is a marked enzyme of granulocyte, is increased by SAM treatment as well. The morphologic observation shows that differentiated HL 60 cells induced by SAM reveal obvious change in morphology of nuclei. All these demonstrate that SAM could induce differentiation of HL 60 cells along granulocyte lineage.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第6期826-829,共4页
Journal of Beijing Normal University(Natural Science)
