摘要
目的鉴定2013年山东省济宁市1例霍乱疫情的分离菌株,掌握其耐药性及病原学特征。方法患者水样便、锅具涂抹物等样本经増菌培养后接种平板进行分离培养,对分离出的3株疑似菌落进行革兰染色镜检、O1/O139群血清凝集实验、生化鉴定、PFGE分型及毒力基因、耐药相关基因及耐药性检测。结果从患者水样便和锅具涂抹物分离的3株菌均为O139群霍乱弧菌,PFGE条带相似度为100%,与2005年济宁1株O139群霍乱菌株相似度为93.3%。3株菌均含有hlyA、ctxAB、toxR、tcpI、rtxA、tcpA毒力基因和intI1、intI4、sxt耐药基因,均对复方新诺明、卡那霉素及多西环素耐药。结论此次霍乱疫情为环境中长期存在的O139群霍乱弧菌污染锅具引起,毒力和耐药性均较强。
Objective To identify strains isolated from one cholera case in Jining of Shandong Province, China in 2013 and to study their drug resistance and etiological characteristics. Methods Samples from watery diarrhea and cooking utensils were cultured and isolated to get individual bacterial colonies. Gram＇s staining, O1/O139 group serum aggluti- nation and biochemical reaction were performed and virulence genes, antibiotic resistance profiles and related resistance genes were determined. Molecular subtyping was carried out by pulsed-field gel electrophoresis （PFGE）. Results All the 3 strains isolated from watery diarrhea and cooking utensils were identified as V. cholerae O139 strains and their PFGE patterns were exactly identical. Cluster analysis also showed that the PFGE pattern of the 3 strains was similar to that of one V. cholerae O139 strain separated from Jining in 2005 with a homology of 93.3%. The 3 strains harbored virulence genes, hlyA, ctxAB, toxR, tcpl, rtxA and tcpA, and antibiotic-resistance genes, intll, intl4 and sxt. Besides, they were all resistant to trimethoprim-sulfamethoxazole, kanamycin and doxycycline, and sensitive to other 8 kinds of antibiotics. Conclusion This cholera was caused by infection of V. cholerae O139 strain with a strong virulence and multi-drug resistance from cooking utensils. Strains of V. cholerae exist in environment all the time, so prevention and control of cholera still need consistent efforts.
出处
《山东大学学报（医学版）》
CAS
北大核心
2014年第2期97-100,105,共5页
Journal of Shandong University:Health Sciences
基金
国家科技重大专项(2012ZX10004215)
国家自然科学基金青年科学基金(81202260)
关键词
霍乱
毒力
耐药
脉冲场凝胶电泳
Cholera
Virulence
Antibiotic resistance
Pulsed-field gel electrophoresis
