摘要
从人胎脑cDNA文库中克隆到一种新的RabcDNA ,全长 92 0bp ,拟编码 2 13个氨基酸残基 ,该蛋白预测的分子质量为 2 45 6 7u ,等电点 7.34,经同源比较 ,该cDNA与GenBank数据库中登录号为X1496 4的Rab蛋白有 83 %的相似性和 76 %的相同性 .将该cDNA克隆到经改造的PBV2 2 0表达质粒 ,转化DH5α菌株诱导表达出该蛋白 .取 2 4种不同组织的总cDNA各 10 0ng ,用该基因序列设计引物作PCR ,结果在胎肝组织中检测到有明显条带 ,表明该Rab基因相对在胎肝有高表达 .
A cDNA clone coding for human Rab protein has been isolated by screening human 18 weeks embryo brain cDNA library and sequenced. This new cDNA clone of 920 bp contains an open reading frame of 213 amino acids whose deduced molecule weight is 24 567 u and isoelectronic point is 7.34. The entire amino acid sequence of novel human Rab was 83% similar to that of Rab protein Q14964 in GenBank and their sequence in the GTP binding domain were almost completely identical, predicting that the novel cDNA is for a human Rab isoform. Expression profile analysis has indicated that the human novel Rab gene was high expressed in fatal liver tissue. This novel human Rab gene was expressed in vitro using modified PBV220 expression vector.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2000年第6期688-691,共4页
Journal of Fudan University：Natural Science
基金
上海市现代生物与新药产业发展基金资助项目!(98431912 1)
